Germinação in vitro de Dipteryx alata Vogel (Fabaceae) e proliferação de brotos sob sistema de ventilação convencional e natural

Ciencia Florestal

article

Andreia Alves da Costa Silveira¹  Lívia Cristina da Silva¹  Sérgio Tadeu Sibov² 

[1]Freelance Researcher

[2]Universidade Federal de Goiás

关键词: Baru;    Cerrado;    Plant growth regulators;    In vitro culture;   

DOI  :  10.5902/1980509841400

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Pesquisas Florestais

【 摘 要 】

Dipteryx alata Vogel is a native species of the Cerrado domain with economic potential for use as a timber and food source. The present study aimed to establish an in vitro germination and shoot proliferation protocol for this species for future rooting and acclimatization investigations. Fruits were collected in October 2013. For asepsis and germination, the seeds were divided into two groups: in the first the seed coat was removed and in the second, maintained. Both groups were submitted to asepsis with detergent and 70% alcohol, followed by different concentrations of sodium hypochlorite (NaClO) (2.5% active chlorine) (v/v): 00%; 10%; 50%; and 100%. Two experiments were conducted for in vitro shoot proliferation: conventional lids or natural ventilation. Nodal segments from plants germinated in vitro (two months old) were inoculated with different concentrations of 6-Benzylaminopurine (BAP) (0.00 µM; 4.44 µM; and 11.10 µM) combined with naphthalene acetic acid (NAA) (0.00 µM; 2.69 µM; and 5.37 µM). The seeds were successfully decontaminated, with those exhibiting an intact seed coat and 50% NaClO found to be superior. High shoot proliferation was observed under natural ventilation, with 4.44 µM BAP (average of 4.03 shoots), a 101.50% increase in relation to controls and 43.93% when compared to the best conventional system treatment. On the other hand, this last system produced taller shoots under 2.69 and 5.37 µM of NAA (average of 19.42 and 18.18 mm, respectively), and 2.69 µM of NAA combined with 4.44 µM of BAP (average of 18.46 mm). By contrast, natural ventilation resulted in taller shoots for 5.37 µM of NAA combined with 11.10 µM of BAP (average of 12.81 mm).

【 授权许可】

CC BY-NC