Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source "Turkish DeLight"

Turkish Journal of Biology

article

ATALAY, NECATݹ  AKCAN, ENVER KAMİL¹  GÜL, MEHMET¹  AYAN, ESRA¹  DESTAN, EBRU¹  KUZUCU, FATMA BETÜL ERTEM¹  TOKAY, NURETTİN¹  ÇAKILKAYA, BARI޹  NERGİZ, ZELİŞ¹  USTA, GÖZDE KARAKADIOĞLU¹  KEPCEOĞLU, ABDULLAH¹  YAPICI, İLKİN¹  TOSUN, BİLGE¹  BALDIR, NİLÜFER¹  YILDIRIM, GÜNSELݹ  JOHNSON, J AUSTIN¹  GÜVEN, ÖMÜR¹  SHAFIEI, ALALEH¹  ARSLAN, NAZLI EYLÜL¹  YILMAZ, MERVE¹  KULAKMAN, CAHİNE¹  PAYDOS, SEYİDE SEDA¹  ÇİNAL, SEYNEP SENA¹  ŞABANOĞLU, KARDELEN¹  PAZARÇEVİREN, AYŞEGÜL¹  YILMAZ, AYŞENUR¹  CANBAY, BAŞAK¹  AŞÇI, BENGİSU¹  KARTAL, ESRA¹  TAVLI, SERRA¹  ÇALISEKİ, MEHMET^1,11  GÖÇ, GÜNCE^1,11  MERMER, ARİF³  YEŞİLAY, GAMZE³  ALTUNTAŞ, SEVDE³  TATEISHI, HIROSHI^1,17  OTSUKA, MASAMI^1,17  FUJITA, MIKAKO^1,17  TEKİN, ŞABAN³  ÇİFTÇİ, HALİLİBRAHİM¹  DURDAĞI, SERDAR^2,21  DOĞANAY, GİZEM DİNLER⁴  KARACA, EZGİ^2,22  TÜRKÖZ, BURCU KAPLAN^2,24  KABASAKAL, BURAK VELİ^1,11  KATI, AHMET³  DEMİRCİ, HASAN¹ 

[1] Department of Molecular Biology and Genetics, Faculty of Science, Koç University;Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University;Experimental Medicine Application & Research Center, University of Health Sciences Türkiye;Department of Molecular Biology and Genetics, Faculty of Science and Letters, İstanbul Technical University;Koç University İşbank Center for Infectious Diseases ,(KUISCID), Koç University;Department of Molecular Biology and Genetics, Faculty of Science and Letters, İstanbul Arel University;Department of Molecular Biology and Genetics, Faculty of Science, Bilkent University;Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Yıldız Technical University;Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Boğaziçi University;Department of Bioinformatics and Genetics, Faculty of Engineering and Natural Sciences, Kadir Has University;Turkish Accelerator and Radiation Laboratory ,(TARLA), Ankara University;Department of Molecular Biology, Genetics and Bioengineering, Graduate School of Engineering and Natural Sciences, Sabancı University;Department of Biology, Faculty of Arts and Science, Kocaeli University;Department of Biotechnology, Hamidiye Institute of Health Sciences, University of Health Sciences Türkiye;Department of Molecular Biology and Genetics, Hamidiye Institute of Health Sciences, University of Health Sciences Türkiye;Department of Tissue Engineering, Hamidiye Institute of Health Sciences, University of Health Sciences Türkiye;Medicinal and Biological Chemistry Science Farm Joint Research Laboratory, Faculty of Life Sciences, Kumamoto University;Department of Drug Discovery, Science Farm Ltd.;The Scientific and Technological Research Council of Türkiye ,(TÜBİTAK) Marmara Research Center ,(MAM), Life Sciences;Department of Basic Medical Sciences, Division of Medical Biology, Faculty of Medicine, University of Health Sciences Türkiye;Department of Biophysics, School of Medicine, Bahçeşehir University;İzmir Biomedicine and Genome Center;İzmir International Biomedicine and Genome Institute, Dokuz Eylül University;Department of Food Engineering, Faculty of Engineering, Ege University;School of Biochemistry, University of Bristol;Stanford PULSE Institute, SLAC National Laboratory

关键词: X-ray crystallography;    light source;    structural biology;    atomic resolution;    drug repurposing;    drug development;    structural dynamics;   

DOI  :  10.55730/1300-0152.2637

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.

【 授权许可】

Unknown