Cytoplasmically localized tRNA-derived fragments inhibit translationin Drosophila S2 cells

Turkish Journal of Biology

article

HAMID, SYED MUHAMMAD¹  AKGÜL, BÜNYAMİN² 

[1] Heart Institute, Cedars-Sinai Medical Center, University of California;Department of Molecular Biology and Genetics, İzmir Institute of Technology

关键词: tRF;    tRNA fragments;    translation;    Drosophila;    proliferation;   

DOI  :  10.55730/1300-0152.2610

学科分类：生物科学（综合）

来源: Scientific and Technical Research Council of Turkey - TUBITAK

【 摘 要 】

Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 and XRN1 proteins. Functionally, tRFs, which slightly suppressed proliferation in S2 cells, inhibited translation without any major shift in the polysome profile. These results suggest that 5?-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.

【 授权许可】

Unknown