Metabolomics Analysis of Combretum lanceolatum Roots in the Presence of Its Endophytic Fungi

Journal of the Brazilian Chemical Society

article

Serrano, Lenard¹  Lacerda, Jhuly W. F¹  Moura, Mariana S¹  Ali, Akbar²  Vasconcelos, Leonardo G. de¹  Sousa Junior, Paulo T¹  Bellete, Barbara S³  Soares, Marcos A¹  Vieira, Lucas C. C¹  Sampaio, Olívia M¹ 

[1] Universidade Federal de Mato Grosso;Government College University;Universidade Federal de Lavras

关键词: plant-fungus interaction;    chemical profile;    primary metabolism;    Trichoderma spirale;    Diaporthe phaseolorum;   

DOI  :  10.21577/0103-5053.20220103

学科分类：内科医学

来源: SciELO

【 摘 要 】

The aim of this work was to identify and quantify the metabolites present in the roots of Combretum lanceolatum inoculated with its endophytic fungi. The metabolomics was accomplished using the 1H nuclear magnetic resonance (NMR) spectral data and evaluated via rNMR software and Madison Metabolomics Consortium Database (MMCD). The principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) showed that plants inoculated with Trichoderma spirale (Ts) present differentiation and discrimination over the time compared to control. Seven days after Ts fungal inoculation, 15 metabolites were identified at different concentrations comparing to the control plants. The plants inoculated with Ts fungus present the metabolites spermidine and pantothenate in higher concentrations and 3-hydroxybutyric acid and b-alanine in lower concentrations compared to control plants, indicating any response to biotic stress. These metabolites are involved in various plant processes, including secondary metabolites biosynthesis, energy metabolism and self-defense. Therefore, this work demonstrates the diversification of primary metabolites composition influenced by endophytes inoculation.

【 授权许可】

CC BY