In vivo Administration of Testosterone Propionate in Cattle Analyzed by High Performance Liquid Chromatography-Tandem Mass Spectrometry: An Enzymatic Hydrolysis Study and Drug Abuse Issues

Journal of the Brazilian Chemical Society

article

Rocha, Diego G.¹  Lana, Mary Ane G.¹  Faria, Adriana F.²  Augusti, Rodinei²  Assis, Débora C. S. de² 

[1] Laboratório Federal de Defesa Agropecuária;Universidade Federal de Minas Gerais

关键词: testosterone;    livestock;    animal experimentation;    Box-Behnken design;   

DOI  :  10.21577/0103-5053.20210159

学科分类：内科医学

来源: SciELO

【 摘 要 】

This study involved an animal experiment in which testosterone propionate was intramuscularly injected into four steers, and the ensuing urine samples were analyzed using high-performance liquid chromatography-tandem mass spectrometry. The enzymatic hydrolysis conditions were analyzed using analysis of variance (ANOVA) and response surface methodology employing a 33 Box-Behnken design. A survey study was performed in which 48 urine samples were collected from eight untreated steers to estimate the endogenous levels of testosterone metabolites, and it was found that 17β-testosterone was not detected in the majority of the samples, whereas 17α-testosterone was present in 43 of the 48. Drug abuse was identified within 11 days of 17β-testosterone administration. The ratio between both isomers was also assessed; however, a cut-off limit based on an untreated population could not be estimated due to the absence of the beta isomer. Therefore, there may be a correlation between the exogenous use of testosterone in castrate animals and high levels of 17β-testosterone, although confirmation through further investigation would be required.

【 授权许可】

CC BY