Adverse Effect of Carboplatin on Biochemical Enzymes And Reproductive Hormones In Male Swiss Albino Mice.

Research Journal of Pharmaceutical, Biological and Chemical Sciences

article

Ojo OO¹  Ayinde S¹  Ayegbusi EO¹  Ogunbiyi BT.² 

[1] Department of Biochemistry, Ekiti State University Ado-Ekiti;Department of Biochemistry, Benjamin S. Carson ,(Snr) School of Medicine, Babcock University Illisan.

关键词: Carboplatin;    Melatonin;    Apoptosis;    Reproductive System;    Enzymes;   

DOI  :  10.33887/rjpbcs/2022.13.6.8

学科分类：工程和技术（综合）

来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences

【 摘 要 】

This work was designed to investigate the adverse effect of carboplatin, a platinum coordinationcompound broadly used as a chemotherapeutic agent in the treatment of cancer on male reproductivesystem by measuring some important enzymes and reproductive hormones using a mouse model. Also, anattenuative effect of melatonin was investigated in this study.Swiss albino mice were randomly selectedinto five groups (n=15). Group I received vehicle and served as control, groups II, III and IV received 1, 5and 10mg/kg.wt of carboplatin respectively while group V was co-treated with 10mg/kg.wt of carboplatinand 5mg/kg.wt of Melatonin via intraperitoneal injection. Five animals from each group were sacrificed ato the interval of 6, 12and 24 hr after carboplatin exposure. Testes were stored in 20 C for biochemical andhormonal analyses. Effect of Testosterone (T) and Luteinizing hormones (FSH) was measured by enzymeimmunosorbent assay (ELISA). Effects on apoptosis and vital steroidogenic enzymes were also measured,Data were analyzed by Duncan ANOVA at p<0.05. It was observed that carboplatin leads to significantdecrease in the levels of the anti-oxidative enzymes in a dose-dependent manner with simultaneousincrease in malondialdehyde level. Decrease level of regulatory enzyme of steroidogenesis pathway wasalso measured. Carboplatin leads induction of apoptosis in the testicular tissue of the mice treated withhigh dose of carboplatin. This study gives insight on the adverse effect of carboplatin on male reproductivesystem and possible recovery effect of melatonin.

【 授权许可】

All Rights reserved