Research Journal of Pharmaceutical, Biological and Chemical Sciences | |
Simultaneous Estimation Of Paracetamol And Phenylephrine Hydrochloride In Pharmaceutical Dosage Form By High Performance Liquid Chromatography. | |
article | |
Uma Shanker Maurya1  Anju Goyal.1  | |
[1] Faculty of Pharmacy, B.N. Institute of Pharmaceutical Sciences, Bhupal Nobles’ University | |
关键词: acetonitrile; accuracy; precision; paracetamol; phenylephrine hydrochloride.; | |
DOI : 10.33887/rjpbcs/2022.13.4.8 | |
学科分类:工程和技术(综合) | |
来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences | |
【 摘 要 】
A simultaneous method of phenylephrine hydrochloride and paracetamol was developed. Theseparation was achieved by kromasil C18 column having dimensions of 250 mm x 4.6mm internal diameter,0C with column oven temperature of 30 . Mobile phase was selected acetonitrile: 0.1% ortho phosphoric acidin the ratio of 45:55 with flow rate of 1ml/min. Detection was carried out at 211nm. Retention time ofphenylephrine hydrochloride and paracetamol were found respectively on 1.8 and 2.5. The method wasvalidated as per ICH guidelines with linearity in the range of 0.2-3µg/mL and 20- 300 µg/mL respectivelyof Phenylephrine hydrochloride and Paracetamol. The developed method was found to be precise, accurateand sensitive for simultaneous estimation of phenylephrine hydrochloride and paracetamol inpharmaceutical dosage form.
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RO202307050005669ZK.pdf | 307KB | download |