Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Metformin HCL, Pioglitazone and Gliclazide in Bulk and Formulation.

Research Journal of Pharmaceutical, Biological and Chemical Sciences

article

Jawale Anup R¹  Rane Sachin S¹  Chaudhari Rajesh Y¹  Patil Vijay R.¹ 

[1] T.V.E.S.’s Hon. Loksevak Madhukarrao Chaudhari College of Pharmacy

关键词: Metformin Hcl;    Pioglitazone and Gliclazide;    Validation;    RP-HPLC;    Simultaneous Estimation;   

DOI  :  10.33887/rjpbcs/2022.13.2.7

学科分类：工程和技术（综合）

来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences

【 摘 要 】

A new simple, rapid, precise, and specific assay method was development and validation of RP- HPLC stability indicating method for the simultaneous estimation of metformin HCL, pioglitazone andgliclazide in bulk and formulation. The analysts were separated by using RP HPLC on a RP- vertex C18column (5 µm, 4.6mm, 250 mm). The mobile phase was methanol: acetonitrile: water at 1.0 mL/minsatisfactorily resolved the binary mixture. The UV detector was operated at 228 nm for the determinationof the drugs. Linearity, accuracy and precision were found to be acceptable over the concentration ranges2of 20-100 µg/ml for metformin HCL, 4-12 µg/ml gliclazide and for 3-15 µg/ml pioglitazone with a R 0.9997, 0.9983 and 0.9989 values respectively, in this mixture. The optimized method proved to bespecific, robust and accurate for the quality control in bulk drug and pharmaceutical formulations.

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