Nephrotoxic Effect “Focal Segmental Glomerulosclerosis” In Wister Rats of Ruta chalepensis L Aqueous Extract Used in Traditional Moroccan Pharmacopoeia.

Research Journal of Pharmaceutical, Biological and Chemical Sciences

article

BELLAHMAR Meryem¹  OUAHBI Abdelilah¹  EL BADAOUI Khalid.¹ 

[1] Environment and Health Laboratory, Faculty of Science, Moulay Ismail University

关键词: FSGS;    Nephrotoxic;    subchronic toxicity;    polyuria;    lead;    Wistar Rats.;   

DOI  :  10.33887/rjpbcs/2021.12.1.28

学科分类：工程和技术（综合）

来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences

【 摘 要 】

Kidney damage following the use of a nephrotoxic herb is a serious condition that is often associatedwith significant morbidity and mortality. Because of its rich vascularization (25% of cardiac output), the kidneyis indeed an organ particularly vulnerable to the toxicity of drugs present in the body. In addition, the existenceof a cortico-medullary osmotic gradient promotes the interstitial accumulation of toxic agents in the papillaeand in the medullary zone. Several floristic and ethnobotanical studies of medicinal plants used by theMoroccan population have made it possible to draw up catalogs of some medicinal species used in indigenoustherapeutic recipes. The toxicity of several of them has been frequently reported. Our research on the possiblenephrotoxic effects of the aqueous extract of Ruta Chalepensis L areal part, a medicinal plant commonly usedin traditional Moroccan pharmacopoeia, aims to study their harmful effects on the physiology and function ofthe renal system. Performing a subchronic toxicity study, an evaluation of the toxicity of the aqueous extract ofthe aerial part of Ruta Chalepensis L in single daily doses was carried out in male and female rats in accordancewith OECD guidelines 423 and 407 for chemical testing. As a single dose of 10g/kg in 24h of the aqueous extractof Ruta Chalepensis L areal part administered intraperitoneally during 2 months. A significant difference wasnoted in the mean weight lost in treated males and females. Also mean relative weights of kidney is reduced(p<0.05) in females and males, and mean relative weights of spleen were significantly reduced (p < 0.001) infemales. The urinary volume shows a chronic polyuria mainly in males compared to females. Histologicalsections of the kidneys show segmental lesions (part of the glomerulus) or global lesions (the wholeglomerulus) and focal lesions (some glomerulus), Sclerosis (or fibrosis) is also observed, which is a cicatricialaccumulation of collagenous material replacing the entire destroyed glomerulus (sclerotic glomerulus) or partof the destroyed glomerulus (segmental sclerosis). Subchronic toxicity study of the aqueous extract aerial partof Ruta Chalepensis L in single daily doses of 10g/kg administered intraperitoneally during 2 months induces anephrotoxic effect, a common glomerular pathologies: the Focal segmental glomerulosclerosis (FSGS) in wistarrats under the conditions of our experiment.

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