Research Journal of Pharmaceutical, Biological and Chemical Sciences | |
Cytogenotoxicity Levels In Buccal Mucosal Cells Of Paint Handlers: Micronuclei Induction And Immunocytochemical Profile (p53 And Ki67). | |
article | |
Anulika Obianuju Onyemelukwe1  Peter Uwadiegwu Achukwu1  Nkiruka Chinonyelum Azubuike1  Okechukwu Steven Onwukwe1  Samuel Ifedioramma Ogenyi2  Uzoamaka Charity Maduakor1  Ogechukwu Florence Odo3  Chinenye Esther Okenwa1  Lawrence Ogbonna Odo.1  | |
[1] Department of Medical Laboratory Sciences, Faculty of Health Sciences & Technology, College of Medicine, University of Nigeria;Department of Medical Laboratory Sciences, Faculty of Health Sciences & Technology, College of Health Sciences, Nnamdi Azikiwe University Awka;Department of Histopathology, Enugu State University Teaching Hospital | |
关键词: Painters; micronuclei; buccal cells; occupational health; immunocytochemical; genotoxicty; mutagens.; | |
DOI : 10.33887/rjpbcs/2020.11.4.18 | |
学科分类:工程和技术(综合) | |
来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences | |
【 摘 要 】
Workers who handle paints are often occupationally exposed to potentially harmful substances. Thisstudy assessed the P53 and Ki67 immunocytochemical profile and the frequency of buccal cell nuclearabnormalities in paint handlers occupationally exposed to paint solvents and metals. A total of 251 apparentlyhealthy individuals comprising 51 control subjects, 28 paint sellers, 63 building painters, 109 paint industryworkers were enrolled in the study. Buccal smears were obtained from each participant and were stained usingHaematoxylin and Eosin technique. A total of 1000 cells per individual were scored under light microscopy todetermine the frequencies of micronuclei (MN) and other nuclear abnormalities. Structured questionnaireswere used to obtain relevant demographic and exposure information about the participants.Immunocytochemical methods were used to assess the patterns of immunoreactivity of Ki-67 and p53 genes onthe buccal cells. All paint handlers had significantly increased frequencies of MN and other nuclearabnormalities. Further analysis showed that the number of years spent in the occupation, alcohol consumptionand cigarette smoking significantly affected the frequency of nuclear abnormalities the among paint handlers. P53 and Ki-67 proteins did not show immunoreaction in the buccal cells of both control and exposed subjects.Paint handlers in Enugu Metropolis may be exposed to substances capable of causing genotoxic changes whichmanifested in their buccal cells as increased occurrence of MN and other nuclear abnormalities.
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