In-vitro studies on biocontrol of Alternaria solani and Botrytis fabae.

Research Journal of Pharmaceutical, Biological and Chemical Sciences

article

Mohamed E. Osman¹  Amany A. Abo Elnasr¹  Walla S.M. Abd El All²  Eslam T. Mohamed.¹ 

[1] Department of Botany and Microbiology, Faculty of Science, Helwan University;National Organization for Drug Control and Research

关键词: Antifungal;    Actinomycetes;    Streptomyces nodosus;    Alternaria solani;    Botrytis fabae.;   

DOI  :  10.33887/rjpbcs/2020.11.2.12

学科分类：工程和技术（综合）

来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences

【 摘 要 】

Thirty-two isolates of actinomycetes were isolated from tomato field infected with early blightdisease at Giza Governorate, Egypt. The isolates were screened for the production of antifungal compoundsagainst Alternaria solani and Botrytis fabae using Agar plug diffusion method. Three Streptomyces strains werethe most potent for active metabolites production on starch nitrate medium. They were identified to beStreptomyces recifensis, Streptomyces gelaticus and Streptomyces nodosus. Streptomyces nodosus was themost potent antimicrobial producer strain. Highly active metabolites were extracted from Streptomycesnodosus. The active metabolite has been identified according to physicochemical characteristics. Elemental 1 analysis (C, H, N, O & S), spectroscopic characteristics (UV absorbance, HPLC, FT-IR, H NMR and Massspectrum), melting point, solubility and color have been investigated. These analyses indicate a suggestedempirical formula of C47H73NO17. The purified antifungal agent shows high level of identity to the knownantibiotic amphotericin B. The minimum inhibition concentrations of the purified antifungal agent were alsodetermined.

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