Improvement of synthetic dyes decolorization by immobilized laccase from Penicillium sp. MN749552.1.

Research Journal of Pharmaceutical, Biological and Chemical Sciences

article

Reyad Mohamed El-Sharkawy¹  Hamed Mohamed El-Shora.² 

[1] Botany and Microbiology Department, Faculty of Science, Benha University;Botany Department, Faculty of Science, Mansoura University

关键词: Laccase;    Penicillium sp.;    Decolorization;    Immobilization;    Redox mediator.;   

DOI  :  10.33887/rjpbcs/2020.11.1.22

学科分类：工程和技术（综合）

来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences

【 摘 要 】

The discharges of various dyes throughout industrial wastes are toxic to the environment. Thetransformation and decolorization of dyes by fungal laccases is an alternative method for dyes detoxificationbefore their release into environment. Thus, laccase from Penicillium sp. MN749552.1 was purified tohomogeneity (50 kDa) and immobilized onto chitin for application in dyes transformation. The immobilizedenzyme exhibited appreciable immobilization efficiency. Laccase was applied for decolorization of numeroussynthetic dyes linked to the chromophore groups of diazo, heterocyclic, anthraquinone and triarylmethane. Atthe corresponding absorbance maxima of each dye, there was a reduction in absorbance peak after beingtreated with free and immobilized laccase. Remazol brilliant blue R (RBBR) and malachite green (MG) displayedpeak shift toward a shorter wavelength, compared to non-degraded dye. The enzymatic decolorizationstrategy of RBBR, MB and MG was improved in the presence of pyrogallol as mediator. Congo red (CR) dye wasnot effectively decolorized by the immobilized enzyme. The addition of laccase redox mediator improved thedecolorization process. The most efficient decolorization process by immobilized laccase was at pH 5.0 and 30°C. The chitin immobilized laccase exhibited good stability during 6 repeated cycles in MG decolorization.These results showed the efficient potentiality of immobilized laccase from Penicillium sp. MN749552.1 in thedecolorization process of the four synthetic dyes from their aqueous solutions.

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