Comparative In vitro Anti Inflammatory Study on Alpha Amyrin Isolated from Bark Extracts of Ficus benghalensis. Linn and Alstonia boonei. De Wild

RGUHS Journal of Pharmaceutical Sciences

article

Ratna Baburaj¹  Rajendra V Sandur¹  Kuntal Das² 

[1] Department of Pharmacology, Krupanidhi College of Pharmacy;Department of Pharmacognosy, Krupanidhi College of Pharmacy

关键词: Ficus benghalensis;    Alstonia boonei;    Alpha amyrin;    Inflammation;    Albumin denaturation;    Anti-proteinase;    Anti-lipoxygenase;   

DOI  :  10.26463/rjps.12_4_3

学科分类：药学、药理学、毒理学（综合）

来源: Rajiv Gandhi University of Health Sciences

【 摘 要 】

Background: Ficus benghalensis and Alstonia boonei are two good sources of triterpenes like alpha amyrin and other bioactive compounds possessing anti-inflammatory activity.Objective: The present study was conducted to compare the potential in vitro anti-inflammatory activity of alpha amyrin isolated from methanolic stem bark extracts of Ficus benghalensis Linn (Fb) and Alstonia boonei. De Wild (Ab) and to identify a better source for isolation of alpha amyrin.Methods: The compound alpha amyrin was identified by Thin layer chromatography (TLC) method, followed by Liquid chromatography mass spectrometry (LCMS) and Fourier transform infrared (FTIR) studies of the extracts and with High performance liquid chromatography (HPLC) determination. Furthermore, the compound was studied for its in vitro anti-inflammatory effect by testing its role on inhibition of albumin denaturation, anti-proteinase and anti-lipoxygenase activities and the results were compared with standard aspirin (100 µg/ mL).Results: The results showed dose dependent activity in all the three in vitro models but showed non-significant variation among the isolated alpha amyrin from two different plants.Conclusion: Amount of isolated alpha amyrin was higher in Fb source (0.42 mg) than Ab plant (0.23 mg) and hence Fb was identified as a better source for isolation of alpha amyrin for further investigations. Background: Ficus benghalensis and Alstonia boonei are two good sources of triterpenes like alpha amyrin and other bioactive compounds possessing anti-inflammatory activity.Objective: The present study was conducted to compare the potential in vitro anti-inflammatory activity of alpha amyrin isolated from methanolic stem bark extracts of Ficus benghalensis Linn (Fb) and Alstonia boonei. De Wild (Ab) and to identify a better source for isolation of alpha amyrin.Methods: The compound alpha amyrin was identified by Thin layer chromatography (TLC) method, followed by Liquid chromatography mass spectrometry (LCMS) and Fourier transform infrared (FTIR) studies of the extracts and with High performance liquid chromatography (HPLC) determination. Furthermore, the compound was studied for its in vitro anti-inflammatory effect by testing its role on inhibition of albumin denaturation, anti-proteinase and anti-lipoxygenase activities and the results were compared with standard aspirin (100 µg/ mL).Results: The results showed dose dependent activity in all the three in vitro models but showed non-significant variation among the isolated alpha amyrin from two different plants.Conclusion: Amount of isolated alpha amyrin was higher in Fb source (0.42 mg) than Ab plant (0.23 mg) and hence Fb was identified as a better source for isolation of alpha amyrin for further investigations.

【 授权许可】

CC BY