Formulation and Evaluation of Gel Containing Anthocyanin of Punica Granatum L. Extract for Anti-Inflammatory and Anti-Microbial Activity

RGUHS Journal of Pharmaceutical Sciences

article

Anita R Desai¹  Laxman S Vijapur¹  Sachin R Teradale¹  Madhushree¹ 

[1] Department of Pharmaceutics, BVVS Hanagal Shri Kumareshwar College of Pharmacy

关键词: Anthocyanin;    Punica granatum L;    Anti-inflammatory;    Anti-microbial;    Topical gel;   

DOI  :  10.26463/rjps.12_3_2

学科分类：药学、药理学、毒理学（综合）

来源: Rajiv Gandhi University of Health Sciences

【 摘 要 】

Aim: The study aimed to formulate and evaluate gel containing anthocyanin extract and to study its anti-inflammatory and anti-microbial activity.Methodology: Anthocyanin was extracted from Punica granatum L. by placing fresh arils in methanol containing 1% HCl (v/v) by maceration method for 24h. Anthocyanin gels were formulated using various polymers like xanthan gum, Carbopol-940, HPMC K100M, and Sodium CMC. Formulated gels were subjected to compatibility studies by ATR, pH, viscosity, spreadability, extrudability, and in-vitro drug release. The best formulations were evaluated for antimicrobial activity which was carried out by the modified disk diffusion method and anti-inflammatory activity by the rat paw edema method.Results: Compatibility studies revealed no interaction of anthocyanin concentrate with the excipients. Formulated gels had a pH similar to skin. Gels prepared using Sodium CMC were less viscous and showed good spreadability and excellent extrudability compared to other formulations. Formulation F7 showed the best result when compared with the marketed diclofenac gel for anti-inflammatory activity. Both gram-positive and gram-negative bacteria were inhibited by anthocyanin concentrate gels. F7 was found to have a substantial anti-inflammatory effect among prepared gels containing anthocyanin concentrate..Conclusion: Anthocyanin extract of Punica granatum L. gel can be utilized to reduce inflammation and can be used to prevent the growth of microorganisms.  Aim: The study aimed to formulate and evaluate gel containing anthocyanin extract and to study its anti-inflammatory and anti-microbial activity.Methodology: Anthocyanin was extracted from Punica granatum L. by placing fresh arils in methanol containing 1% HCl (v/v) by maceration method for 24h. Anthocyanin gels were formulated using various polymers like xanthan gum, Carbopol-940, HPMC K100M, and Sodium CMC. Formulated gels were subjected to compatibility studies by ATR, pH, viscosity, spreadability, extrudability, and in-vitro drug release. The best formulations were evaluated for antimicrobial activity which was carried out by the modified disk diffusion method and anti-inflammatory activity by the rat paw edema method.Results: Compatibility studies revealed no interaction of anthocyanin concentrate with the excipients. Formulated gels had a pH similar to skin. Gels prepared using Sodium CMC were less viscous and showed good spreadability and excellent extrudability compared to other formulations. Formulation F7 showed the best result when compared with the marketed diclofenac gel for anti-inflammatory activity. Both gram-positive and gram-negative bacteria were inhibited by anthocyanin concentrate gels. F7 was found to have a substantial anti-inflammatory effect among prepared gels containing anthocyanin concentrate..Conclusion: Anthocyanin extract of Punica granatum L. gel can be utilized to reduce inflammation and can be used to prevent the growth of microorganisms.

【 授权许可】

CC BY