Preliminary Phytochemical Screening and Antibacterial Activity of Leaves ofPassiflora foetida Linn.

RGUHS Journal of Pharmaceutical Sciences

article

Akila E¹  Mohammed Haroon² 

[1] Department of Pharmacognosy, RR College of Pharmacy;Department of Pharmacognosy, Madras Medical College

关键词: Passiflora foetida;    Antibacterial studies;    Inhibitory activity;    Microorganism;   

DOI  :  10.26463/rjps.12_2_5

学科分类：药学、药理学、毒理学（综合）

来源: Rajiv Gandhi University of Health Sciences

【 摘 要 】

Background: Passiflora foetida is widely used in Indian traditional system of medicines to cure various human diseases.Aim: The present study envisaged to select an appropriate local plant with anti-bacterial ability based on its folklore claim and to scientifically establish its anti-bacterial activity.Methods: Shade dried powdered material of leaves of Passiflora foetida Linn., was used for the determination of physiochemical constants in accordance with WHO guidelines. Subsequent solvent extracts were prepared using petroleum ether, chloroform, ethanol and water. Each extract was further proceeded for detailed phytochemical study and anti-bacterial screening.Results: The preliminary phytochemical screening revealed the chemical constituents of alkaloids, flavonoids and phenolic compounds. In vitro antibacterial studies on the leaf extracts were carried out on medically important micro-organisms such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Staphylococcus epidermis and Pseudomonas aeruginosa. Growth of all cultures was seen in the solvent control and in positive control. Petroleum ether and chloroform extracts showed inhibition of growth of microorganisms like Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Staphylococcus epidermis at 200 µg/mL. No growth was seen for Pseudomonas aeruginosa at the concentration of 300 µg/mL. Ethanol and aqueous extracts exhibited inhibitory effect on the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Staphylococcus epidermis at 200 µg/mL and Pseudomonas aeruginosa at the concentration of 100 µg/mL. A dose dependent inhibitory activity was observed.Conclusion: From the above findings, it was clear that the secondary metabolites present in leaf extracts of Passiflora foetida Linn are the source of plant-derived antimicrobial substances. Background: Passiflora foetida is widely used in Indian traditional system of medicines to cure various human diseases.Aim: The present study envisaged to select an appropriate local plant with anti-bacterial ability based on its folklore claim and to scientifically establish its anti-bacterial activity.Methods: Shade dried powdered material of leaves of Passiflora foetida Linn., was used for the determination of physiochemical constants in accordance with WHO guidelines. Subsequent solvent extracts were prepared using petroleum ether, chloroform, ethanol and water. Each extract was further proceeded for detailed phytochemical study and anti-bacterial screening.Results: The preliminary phytochemical screening revealed the chemical constituents of alkaloids, flavonoids and phenolic compounds. In vitro antibacterial studies on the leaf extracts were carried out on medically important micro-organisms such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Staphylococcus epidermis and Pseudomonas aeruginosa. Growth of all cultures was seen in the solvent control and in positive control. Petroleum ether and chloroform extracts showed inhibition of growth of microorganisms like Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Staphylococcus epidermis at 200 µg/mL. No growth was seen for Pseudomonas aeruginosa at the concentration of 300 µg/mL. Ethanol and aqueous extracts exhibited inhibitory effect on the growth of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Staphylococcus epidermis at 200 µg/mL and Pseudomonas aeruginosa at the concentration of 100 µg/mL. A dose dependent inhibitory activity was observed.Conclusion: From the above findings, it was clear that the secondary metabolites present in leaf extracts of Passiflora foetida Linn are the source of plant-derived antimicrobial substances.

【 授权许可】

CC BY