Pharmacognostical Evaluation and In vitro Cytotoxic Activity of Alcoholic and Aqueous Extracts of Corm of Amorphophallus paeoniifolius (Dennst)

RGUHS Journal of Pharmaceutical Sciences

article

Rajesh Avadhoot Shastry¹  Naveen Karur¹  PrasannaVasantrao Habbu¹  Smita Daniel Madagundi¹  Basavaraj Sangangouda Patil¹  Venkatrao Hanumanthrao Kulkarni¹ 

[1] PG Department of Pharmacognosy, S.E.T’s College of Pharmacy

关键词: Amorphophallus paeoniifolius;    cytotoxicity;    MTT assay;    sulforhodamine B (SRB) assay;    Trypan blue assay;   

DOI  :  10.26463/rjps.11_4_6

学科分类：药学、药理学、毒理学（综合）

来源: Rajiv Gandhi University of Health Sciences

【 摘 要 】

Background : Cancer is an abnormal growth of cells that can arise in any part of the body and causes death. It affects more than one-third in the world and is 20% higher in all fatalities. Tobacco, viral infection, chemicals, radiation etc. causes cancer. Several cancer treatments include surgery, chemotherapy, and radiotherapy.Objective: In this investigation, a corm of Amorphophallus paeoniifolius (AP) was evaluated for pharmacognostical, preliminary phytochemical investigation and curative effect of aqueous extract of Amorphophallus paeoniifolius (APA) and alcoholic extract of Amorphophallus paeoniifolius (APE) corm for in vitro anti-cancer activity.Methodology : In the present study, breast cancer, cancer of prostate and cancer of cervical human cell lines were used by using MTT, SRB and Trypan blue methods followed by their histopathological studies.Results : The pharmacognostical studies helps in authentication and identification of plant. The results of cytotoxicity studies of aqueous extract of corm of AP revealed significant cytotoxic properties as compared to alcoholic extract and control group against breast carcinoma, prostate carcinoma, carcinoma of cervical cell lines in all the tested in vitro anti-cancer models with CTC50 value 500 μg/mL. The % inhibition depends on the increase in concentration of crude extracts and attains linearity till it reaches CTC50 value.Conclusion: From the results, we can conclude that aqueous and alcoholic extracts of corm of plant can be employed in treatment of various cancer diseases. Background : Cancer is an abnormal growth of cells that can arise in any part of the body and causes death. It affects more than one-third in the world and is 20% higher in all fatalities. Tobacco, viral infection, chemicals, radiation etc. causes cancer. Several cancer treatments include surgery, chemotherapy, and radiotherapy.Objective: In this investigation, a corm of Amorphophallus paeoniifolius (AP) was evaluated for pharmacognostical, preliminary phytochemical investigation and curative effect of aqueous extract of Amorphophallus paeoniifolius (APA) and alcoholic extract of Amorphophallus paeoniifolius (APE) corm for in vitro anti-cancer activity.Methodology : In the present study, breast cancer, cancer of prostate and cancer of cervical human cell lines were used by using MTT, SRB and Trypan blue methods followed by their histopathological studies.Results : The pharmacognostical studies helps in authentication and identification of plant. The results of cytotoxicity studies of aqueous extract of corm of AP revealed significant cytotoxic properties as compared to alcoholic extract and control group against breast carcinoma, prostate carcinoma, carcinoma of cervical cell lines in all the tested in vitro anti-cancer models with CTC50 value > 500 μg/mL. The % inhibition depends on the increase in concentration of crude extracts and attains linearity till it reaches CTC50 value.Conclusion: From the results, we can conclude that aqueous and alcoholic extracts of corm of plant can be employed in treatment of various cancer diseases.

【 授权许可】

CC BY