Validation of RP-HPLC Method and UV Spectrophotometric Method for the Quantitative Determination of Aztreonam in Bulk and Pharmaceutical Dosage Form

RGUHS Journal of Pharmaceutical Sciences

article

Sayani Bhattacharyya¹  Preethi Sudheer and Bharani S Sogali¹ 

[1]Department of Pharmaceutics, Krupanidhi college of Pharmacy

关键词: Aztreonam;    RP-HPLC method;    UV spectrophotometric method;    Analytical validation.;   

DOI  :  10.5530/rjps.2018.4.2

学科分类：药学、药理学、毒理学（综合）

来源: Rajiv Gandhi University of Health Sciences

【 摘 要 】

The present study focuses on the development of rapid and sensitive development of RP- HPLC method with UV detection and spectrophotometric method for routine analysis of aztreonam in bulk and pharmaceutical dosage form. Chromatographic method was developed in C-8 Phenomenex HPLC column with a mobile phase methanol: phosphate buffer pH 5.4 (70:30) at a flow rate of 1ml/min at 300 nm. The assay showed linearity in the range of 2-10 µg/ml with a correlation coefficient of 0.9999. The UV spectrophotometric method was performed at 293 nm in phosphate buffer solution of pH 7.4. The correlation coefficient was found to be 0.9989. Both the processes were found to be sensitive, rapid, precise, accurate and robust. Therefore, it can be concluded that both the processes can be used for routine quality control test of aztreonam in bulk and pharmaceutical preparation. The present study focuses on the development of rapid and sensitive development of RP- HPLC method with UV detection and spectrophotometric method for routine analysis of aztreonam in bulk and pharmaceutical dosage form. Chromatographic method was developed in C-8 Phenomenex HPLC column with a mobile phase methanol: phosphate buffer pH 5.4 (70:30) at a flow rate of 1ml/min at 300 nm. The assay showed linearity in the range of 2-10 µg/ml with a correlation coefficient of 0.9999. The UV spectrophotometric method was performed at 293 nm in phosphate buffer solution of pH 7.4. The correlation coefficient was found to be 0.9989. Both the processes were found to be sensitive, rapid, precise, accurate and robust. Therefore, it can be concluded that both the processes can be used for routine quality control test of aztreonam in bulk and pharmaceutical preparation.

【 授权许可】

CC BY