CHMP4C regulates lung squamous carcinogenesis and progression through cell cycle pathway

Journal of Thoracic Disease

article

Bo Liu¹  Sixuan Guo²  Geng-Hui Li³  Yue Liu⁴  Xu-Zhi Liu¹  Jian-Bo Yue¹  Hong-Yan Guo⁵ 

[1] The Third Affiliated Hospital of Qiqihar Medical College Department of Respiratory and Critical Care Medicine;The Second Clinical College, Medical College of Nanchang University;Geriatric Department of The First Hospital of Qiqihar;The Third Affiliated Hospital of Qiqihar Medical College Department of Pharmacy;The Qiqihar Medical College Department of Biochemistry

关键词: Lung squamous cell carcinoma (LUSC);    CHMP4C;    survival prognosis;    cell cycle;   

DOI  :  10.21037/jtd-21-583

学科分类：呼吸医学

来源: Pioneer Bioscience Publishing Company

【 摘 要 】

Background: Lung cancer is a common kind of human malignancies. Lung squamous cell carcinoma (LUSC) is a key subtype of lung cancer. Cell cycle plays an important role in the development and occurrence of LUSC, however, there is still a lack of cell cycle-related genes in LUSC diagnosis and prediction of prognosis. Methods: We identified differentially expressed genes (DEGs) with “limma” package in R software, and determined the biomarkers of LUSC in diagnosing by performing receiver operating characteristic (ROC) curve analysis, the biomarker effectiveness in diagnosing LUSC was assessed by performing five-fold cross-validation with logistic regression. Kaplan-Meier plot and the nomogram assessed the relationship between the biomarker and patient survival, and WB and qRT-PCR detected the biomarker expression in cells and tissues. Flow cytometry detects the role of the biomarker in the cell cycle. Results: Integration analysis with The Cancer Genome Atlas (TCGA) database obtained a unique gene related to cell cycle in LUSC (Charged multivesicular body protein 4C, CHMP4C), and the protein of CHMP4C was highly expressed in LUSC tissues. ROC analysis indicated that CHMP4C was a biomarker for the diagnosis of LUSC. Bioinformatic analysis indicated that CHMP4C might be associated with cell cycle in LUSC. CHMP4C knockdown resulted in S-phase arrest of cells with LUSC. According to the survival rate analysis, CHMP4C overexpression indicated poor prognosis in patients with LUSC. Conclusions: CHMP4C regulates the proliferation process of tumor cells through the cell cycle. It can be used as a potential diagnostic and prognostic biomarker for LUSC.

【 授权许可】

Unknown