Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens

Bioscience of Microbiota, Food and Health

article

Amany RAMAH¹  Masahiro YASUDA¹  Yuki OHASHI¹  Shoichiro IMATAKE¹  Noriko IMAIZUMI²  Tetsuo KIDA²  Tenya YANAGITA²  Ryoko UEMURA⁴  Mahmoud BAAKHTARI¹  Hatem H. BAKRY³  Nabila M. ABDELALEEM³  Elham A EL-SHEWY³ 

[1] Graduate School of Medicine and Veterinary Medicine, University of Miyazaki;Laboratory of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki;Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University;Laboratory of Animal Health, Faculty of Agriculture, University of Miyazaki;Faculty of Veterinary Science, Balkh University

关键词: chicken;    growth performance;    immunity;    probiotics;    tannin;   

DOI  :  10.12938/bmfh.2021-058

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

Tannins (TAs) are an anti-nutritional substance commonly used as a natural feed additive for livestock. However, our previous study described the dose-dependent adverse effects of TA on immune responses and growth in chickens. In this study, we evaluated the protective effects of a probiotic preparation (BT) consisting of three different bacteria (Bacillus mesenteric, Clostridium butyricum, and Streptococcus faecalis) against TA-induced immunosuppression in chickens. Forty chicks were divided into 4 groups as follows: the CON group (basal diet), BT group supplemented with 3 g BT/kg diet, tannic acid (TA) group supplemented with 30 g TA/kg diet, and BT+TA group supplemented with 3 g BT/kg diet + 30 g TA/kg diet. The feeding trial lasted for 35 days. Lymphocyte subset, macrophage phagocytosis, cytokine mRNA expression, and primary and secondary IgY immune responses were evaluated. BT supplementation significantly improved TA-induced reductions in final body weight, body weight gain, feed intake, and relative weights of lymphoid organs compared with the TA group. Furthermore, in the spleen and cecal tonsil (CT), the relative populations of CD4+, CD8+, and CD4+CD8+ cells in the BT+TA group were significantly ameliorated compared with the TA group. Additionally, comparison with the TA group showed that the chickens in the BT+TA group had an improved relative population of B cells in the CT and that macrophage phagocytosis in the spleen was significantly increased. Chickens in the BT+TA group showed significant increases in IFN-γ and IL-4 mRNA expression in the spleen compared with the TA group. The primary and secondary IgY responses were significantly improved. These results revealed that supplementation with BT protects against TA-induced immunosuppression in chickens.

【 授权许可】

CC BY