Iranian Journal of Biotechnology | |
Insights Into The Effects of Amino Acid Substitutions on The Stability of Reteplase Structure: A Molecular Dynamics Simulation Study | |
article | |
Haji-Allahverdipoor, Kaveh1  Jalali Javaran, Mokhtar2  Rashidi Monfared, Sajad2  Khadem-erfan, Mohammad Bagher1  Nikkhoo, Bahram1  Bahrami Rad, Zhila1  EslamI, Habib3  Nasseri, Sherko1  | |
[1] Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences;Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University;Department of Pharmacology and Toxicology, School of Pharmacy, Hormozgan University of Medicinal sciences | |
关键词: Amino Acid Substitution; Homology Modeling; Molecular Dynamics Simulation; Protein Stability; Reteplase; Recombinant plasminogen activator; | |
DOI : 10.30498/ijb.2022.308798.3175 | |
学科分类:生物技术 | |
来源: National Institute for Genetic Engineering and Biotechnology | |
【 摘 要 】
Background: Reteplase (recombinant plasminogen activator, r-PA) is a recombinant protein designed to imitate the endogenous tissue plasminogen activator and catalyze the plasmin production. It is known that the application of reteplase is limited by the complex production processes and protein’s stability challenges. Computational redesign of proteins has gained momentum in recent years, particularly as a powerful tool for improving protein stability and consequently its production efficiency. Hence, in the current study, we implemented computational approaches to improve r-PA conformational stability, which fairly correlates with protein’s resistance to proteolysis. Objectives: The current study was developed in order to evaluate the effect of amino acid substitutions on the stability of reteplase structure using molecular dynamic simulations and computational predictions. Materials and Methods: Several web servers designed for mutation analysis were utilized to select appropriate mutations. Additionally, the experimentally reported mutation, R103S, converting wild type r-PA into non-cleavable form, was also employed. Firstly, mutant collection, consisting of 15 structures, was constructed based on the combinations of four designated mutations. Then, 3D structures were generated using MODELLER. Finally, 17 independent 20-ns molecular dynamics (MD) simulations were conducted and different analysis were performed like root-mean-square deviation (RMSD), root-mean-square fluctuations (RMSF), secondary structure analysis, number of hydrogen bonds, principal components analysis (PCA), eigenvector projection, and density analysis. Results: Predicted mutations successfully compensated the more flexible conformation caused by R103S substitution, so, improved conformational stability was analyzed from MD simulations. In particular, R103S/A286I/G322I indicated the best results and remarkably enhanced the protein stability. Conclusion: The conformational stability conferred by these mutations will probably lead to more protection of r-PA in protease-rich environments in various recombinant systems and potentially enhance its production and expression level.
【 授权许可】
Unknown
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