Comparative Analysis of the Interaction of Cytochrome C with Supported Lipid Films and DNA Aptamers Using QCM-D Method

Engineering Proceedings

article

Marek Tatarko¹  Sandro Spagnolo¹  Martin Csiba¹  Veronika Šubjaková¹  Tibor Hianik¹ 

[1] Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava

关键词: cytochrome c;    biosensor;    lipid films;    DNA aptamers;    QCM-D;   

DOI  :  10.3390/IECB2023-14752

来源: mdpi

【 摘 要 】

Cytochrome c (cyt c) is an important indicator of cell apoptosis and can, therefore, be used for the diagnosis of cancer. We performed a comparative analysis of cyt c detection on the surface of lipid films or a monolayer of 11-mercaptoundecanoic acid (MUA) with immobilized specific or nonspecific DNA aptamers. A quartz crystal microbalance with dissipation monitoring (QCM-D) in a multiharmonic mode was used to study the interaction of cyt c with various surfaces. For this purpose, changes in the resonant frequency, Δf, and dissipation, ΔD, were determined. The strongest interaction of cyt c was observed with sensors based on specific DNA aptamers that were accompanied by a decrease in frequency and an increase in dissipation. The limit of detection (LOD) for this aptasensor was established as 2.89 ± 0.12 nM. The interaction of cyt c with supported lipid films also resulted in a decrease in resonant frequency, but significant changes occurred only in the µM concentration range of cyt c. Changes in dissipation were much lower in comparison with aptamer-based surfaces, which suggests a weaker contribution of cyt c adsorption to the viscosity.

【 授权许可】

Unknown