Immunosensing Cancer Markers through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates

Engineering Proceedings

article

Georgia Geka¹  Anastasia Kanioura¹  Ioannis Kochylas³  Vlassis Likodimos³  Spiros Gardelis³  Kalliopi Chatzantonaki⁴  Ekaterina Charvalos⁴  Anastasios Dimitriou⁵  Nikolaos Papanikolaou⁵  Anastasios Economou²  Sotirios Kakabakos¹  Panagiota Petrou¹ 

[1] Immunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, National Centre for Scientific Research “Demokritos”;Department of Chemistry, National and Kapodistrian University of Athens;Section of Condensed Matter Physics, Department of Physics, National and Kapodistrian University of Athens;Molecular Diagnosis Department, Invitrolabs S.A.;Institute of Nanoscience & Nanotechnology, National Centre for Scientific Research “Demokritos”

关键词: optical biosensor;    ovarian cancer;    CA125;    HE4;    immunochemical detection;    photoluminescence;   

DOI  :  10.3390/IECB2023-14583

来源: mdpi

【 摘 要 】

Noble metal nanostructured substrates enhance photoluminescence emitted from molecules immobilized onto their surface, allowing for the development of highly sensitive immunoassays employing fluorescent labels. In this work, nanostructured silver surfaces were implemented as substrates for the immunochemical detection of two ovarian cancer markers, carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4). Biotinylated detection antibodies were used to allow for the detection of immunocomplexes through a reaction with streptavidin conjugated to Rhodamine Red-X. The detection limits achieved were 2.5 U/mL and 0.06 ng/mL for CA125 and HE4, respectively, with linear dynamic ranges, covering the concentration ranges of both healthy and ovarian cancer patients.

【 授权许可】

Unknown