Target Discovery of Flavonoids from Elymus nutans Griseb Using Medium- and High-Pressure Liquid Chromatography Combined with Online High-Performance Liquid Chromatography–1,1-diphenyl-2-picrylhydrazyl Detection

Separations

article

Lili Sheng¹  Zhaofei Hou¹  Yufeng Hu³ 

[1] Minmetals Salt Lake Co., Ltd.;China Minmetals Co., Ltd.;School of Chemical Engineering and Environment, University of Petroleum

关键词: Elymus nutans Griseb;    preparative isolation;    DPPH inhibitors;    flavonoids;   

DOI  :  10.3390/separations9120437

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Forage-based nutrients constitute the main forage value of forage grass. Elymus nutans Griseb possesses a wide ecological adaptability, enhanced crude protein content, good palatability, and excellent genes. Herein, employing medium- (MPLC) and high-pressure liquid chromatography (HPLC), along with online HPLC-DPPH (OHD)-based identification, two primary radical scavenging compounds were extracted and identified from the methanolic extract of Elymus nutans Griseb. With a starting material of 300 g of Elymus nutans Griseb, 5.95 g of the target DPPH suppressors fraction (Fr6) was separated following one cycle of MCI GEL® 95% purity. This newly designed procedure was highly effective for the targeted flavonoids, and high-purity radical scavenger extraction from forage extracts. This methodology can potentially provide a scientific basis for their quality evaluation.

【 授权许可】

CC BY