Validation of an Analytical Method for the Determination of Thiabendazole in Various Food Matrices

Separations

article

Sun-Il Choi¹  Xionggao Han¹  Se-Jeong Lee¹  Xiao Men¹  Geon Oh¹  Doo-Sik Lee¹  Ok-Hwan Lee¹ 

[1] Department of Food Biotechnology and Environmental Science, Kangwon National University

关键词: thiabendazole;    high-performance liquid chromatography;    method validation;    measurement uncertainty;    food matrices;   

DOI  :  10.3390/separations9060135

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

In many countries, thiabendazole is used as a fungicide to prevent the decay of food and to lengthen storage periods. However, in Korea, thiabendazole is unauthorised and does not have standards or specifications for use as a food additive. In this study, a simple analytical method was developed and validated using the HPLC–PDA method to detect thiabendazole in foods frequently consumed in South Korea. The calibration curve was obtained using samples of solid and liquid foods containing banana and citrus fruits containing concentrations in the range of 0.31–20.00 μg/mL with a satisfactory coefficient of determination (R2) of 0.999. The limit of detection (LOD) values for the solid and liquid food samples were 0.009 and 0.017 μg/mL, respectively, and the limit of quantification (LOQ) values were 0.028 and 0.052 μg/mL. The intra-day and inter-day precision values were less than 1.33% (relative standard deviation), and the recoveries of thiabendazole from spiked solid and liquid food samples ranged from 93.61 to 98.08% at concentration levels of 2.5, 5, and 10 μg/mL. In addition, the expanded uncertainties of the measu-rements ranged from 0.57 to 3.12%. These results showed that the developed method was appropriate for the quantitative analysis of thiabendazole in solid and liquid foods containing banana and citrus fruits.

【 授权许可】

CC BY