Enhanced Sample Throughput Capillary Zone Electrophoresis with UV Detection in Hydrodynamically Closed System for Determination of Ibuprofen

Separations

article

Ondrej Stefanik¹  Andrea Horniakova¹  Ivana Cizmarova¹  Michaela Matuskova¹  Veronika Mikusova²  Peter Mikus¹  Juraj Piestansky¹ 

[1]Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava

[2]Department of Galenic Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava

[3]Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava

关键词: capillary zone electrophoresis;    repeated sample injection;    hydrodynamically closed separation system;    ultraviolet detection;    ibuprofen;    analytical method evaluation;    drug quality control;   

DOI  :  10.3390/separations9050118

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

A simple analytical approach based on capillary zone electrophoresis with ultraviolet detection and repeated sample injection strategy (applied in a hydrodynamically closed separation system for the first time) was developed for the determination of ibuprofen (IBU) in commercially available pharmaceutical preparations. The proposed method was characterized by significantly increased sample throughput and favorable validation parameters, highly demanded in routine quality control laboratories. The limit of detection was predicted at the concentration level of 0.31 µg/mL. Intra-day precision expressed as the relative standard deviation of IBU concentration ranged from 1.9 to 5.6%, and corresponding intra-day accuracy expressed as the relative error was in the interval of 87.1–106.5%. Inter-day precision was in the range of 2.6–15.0%, and inter-day accuracy was 94.9–102.7%. The developed method was able to quantify IBU in complex pharmaceutical matrices represented by commercially available tablets and oral suspension. The determined contents of IBU in the tested dosage forms were in good agreement with the manufacturer’s declaration. The analytical performance of the developed method was evaluated according to the innovative RGB Additive Color Model strategy. It was demonstrated that the proposed method is characterized by very good analytical performance parameters, safety and eco-friendliness, and practical effectiveness.

【 授权许可】

CC BY