Extraction and Characterization of Flavanol-Rich Nutraceuticals Based on High-Performance Liquid Chromatography

Separations

article

Oscar Vidal-Casanella¹  Kevin Arias-Alpizar¹  Oscar Núñez¹  Javier Saurina¹ 

[1] Department of Chemical Engineering and Analytical Chemistry, University of Barcelona;Research Institute in Food Nutrition and Food Safety, University of Barcelona

关键词: flavanols;    medicinal properties;    nutraceuticals;    HPLC-UV/Vis;    HPLC-FLD;    principal component analysis;   

DOI  :  10.3390/separations9040087

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Flavanols are a class of polyphenols that have become the focus of much research because of their medicinal properties. For this paper, a new method to determine polyphenols in nutraceutical samples was developed, based on solid-liquid extraction and further analysis by liquid chromatography. Analytes were recovered by ultrasound-assisted extraction, using a mixture of methanol/water/hydrochloric acid (70:29:1, v:v:v) as the solvent. Reversed-phase high-performance liquid chromatography with ultraviolet/visible (HPLC-UV/Vis) and fluorescence detection (HPLC-FLD) were developed to determine the content of the principal flavanols. The HPLC-FLD method showed better selectivity and sensitivity, so it was selected for further studies. The analytical parameters established with FLD were satisfactory in terms of linearity (R2 ≥ 0.993), repeatability (RSD% ≥ 2.2), and limits of detection of 0.1 mg L−1 or below. The nutraceutical samples were analyzed by HPLC-FLD to determine the compositional profiles of flavanols, then they were subsequently treated using chemometric methods, such as principal component analysis (PCA). Results showed that the study of compositional profiles from the selected flavanols was sufficient for description and discrimination purposes. The cranberry samples were the richest in flavanols, procyanidin A2 being the principal flavanol, and artichoke samples the least rich.

【 授权许可】

CC BY