Assessment of the Risk of Exotic Zika Virus Strain Transmission by Aedes aegypti and Culex quinquefasciatus from Senegal Compared to a Native Strain

Tropical Medicine and Infectious Disease

article

Alioune Gaye¹  Cheikh Fall²  Oumar Faye³  Myrielle Dupont-Rouzeyrol⁴  El Hadji Ndiaye¹  Diawo Diallo¹  Paolo Marinho de Andrade Zanotto⁵  Ibrahima Dia¹  Scott C. Weaver⁶  Mawlouth Diallo¹ 

[1] Pole de Zoologie Médicale, Institut Pasteur de Dakar;Pole de Microbiologie, Institut Pasteur de Dakar;Pole de Virologie, Institut Pasteur de Dakar;URE Dengue et Arboviroses, Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur;Microbiology Department, Institute of Biomedical Sciences, University of São Paulo;World Reference Center for Emerging Viruses and Arboviruses, Institute for Human Infections and Immunity, Department of Microbiology and Immunology, University of Texas Medical Branch

关键词: Zika virus;    A. aegypti;    C. quinquefasciatus;    Brazil;    New Caledonia;    Senegal;   

DOI  :  10.3390/tropicalmed8020130

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Zika virus (ZIKV) shows an enigmatic epidemiological profile in Africa. Despite its frequent detection in mosquitoes, few human cases have been reported. This could be due to the low infectious potential or low virulence of African ZIKV lineages. This study sought to assess the susceptibility of A. aegypti and C. quinquefasciatus to ZIKV strains from Senegal, Brazil, and New Caledonia. Vertical transmission was also investigated. Whole bodies, legs/wings and saliva samples were tested for ZIKV by real-time PCR to estimate infection, dissemination and transmission rates as well as the infection rate in the progeny of infected female A. aegypti. For A. aegypti, the Senegalese strain showed at 15 days post-exposure (dpe) a significantly higher infection rate (52.43%) than the Brazilian (10%) and New Caledonian (0%) strains. The Brazilian and Senegalese strains were disseminated but not detected in saliva. No A. aegypti offspring from females infected with Senegalese and Brazilian ZIKV strains tested positive. No infection was recorded for C. quinquefasciatus. We observed the incompetence of Senegalese A. aegypti to transmit ZIKV and the C. quinquefasciatus were completely refractory. The effect of freezing ZIKV had no significant impact on the vector competence of Aedes aegypti from Senegal, and vertical transmission was not reported in this study.

【 授权许可】

CC BY