An Optimised MS-Based Versatile Untargeted Metabolomics Protocol

Separations

article

Cátia F. Marques¹  Gonçalo C. Justino¹ 

[1] Centro de Química Estrutural–Institute of Molecular Sciences, Instituto Superior Técnico, Universidade de Lisboa

关键词: metabolomics;    mouse;    human;    yeast;    bacteria;    culture cells;    tissues;    plasma;    urine;    faeces;    ESI-UHPLC-MS;   

DOI  :  10.3390/separations10050314

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Untargeted metabolomics approaches require complex samples containing the endogenous metabolites of a biological system. Here, we describe a set of protocols that can be applied to various types of samples, including prokaryotic and eukaryotic cells, as well as animal and human samples. Following a single extraction step, samples are analysed using different chromatographic conditions coupled to high-resolution mass spectrometry. Quantification of metabolite changes between samples is performed without internal standards, using peak areas from extracted ion chromatograms for statistical analysis. Bioinformatics annotation of the results allows a pathway- and process-oriented analysis across biological sample conditions, allowing a complete pathway interrogation.

【 授权许可】

CC BY