Development and Validation of a Stability-Indicating Greener HPTLC Method for the Estimation of Flufenamic Acid

Separations

article

Prawez Alam¹  Faiyaz Shakeel²  Mohammed H. Alqarni¹  Ahmed I. Foudah¹  Tariq M. Aljarba¹  Mohammed M. Ghoneim³  Syed Mohammed Basheeruddin Asdaq³  Sultan Alshehri⁴ 

[1] Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University;Department of Pharmaceutics, College of Pharmacy, King Saud University;Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University;Department of Pharmaceutical Sciences, College of Pharmacy, AlMaarefa University

关键词: AGREE;    flufenamic acid;    greener HPTLC;    stability-indicating;    validation;   

DOI  :  10.3390/separations10010039

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

The literature on ecofriendly/greener high-performance thin-layer chromatographic (HPTLC) methods for quantifying flufenamic acid (FFA) is scant. In order to develop and validate a stability-indicating greener HPTLC densitometry assay for FFA determination in marketed products, this research was conducted. The ecofriendly eluent system was composed of ethanol–water (70:30 v/v). FFA was measured at 290 nm of wavelength. The greenness scale of suggested analytical assay was derived using “Analytical GREENness (AGREE)” methodology. The suggested stability-indicating HPTLC assay was linear for FFA determination in 25–1400 ng/band range with a determination coefficient of 0.9974. The suggested analytical assay for FFA analysis was simple, rapid, accurate, precise, robust, selective, stability-indicating, and greener. The AGREE scale for the developed stability-indicating HPTLC assay was derived to be 0.77 utilizing AGREE methodology, indicating an outstanding greenness characteristic of the suggested densitometry technique. The ecofriendly HPTLC technique was able to detect FFA degradation product under forced degradation studies, indicating its stability-indication characteristics and selectivity. The amount of FFA in marketed tablets brand A and B was determined to be 101.28 and 99.17%, respectively, indicating the suitability of the suggested analytical technique in the assay of FFA in marketed products. These results indicated that FFA in marketed products may be routinely measured using the stability-indicating greener HPTLC technique.

【 授权许可】

CC BY