Raman spectroscopic analysis of DEA 1.1 canine RBCmembrane glyco protein and its application in canineblood typing

Journal of Livestock Science

article

G. Kalaiselvi¹  K.G. Tirumurugaan¹  G. Dhinakar Raj¹  K. Vijayarani¹  R. Baranidharan¹ 

[1] Department of Animal Biotechnology, Madras Veterinary College

关键词: Raman spectroscopy;    Dog erythrocytic membrane antigen;    Amide 1 Bands;    blood grouping;   

DOI  :  10.33259/JLivestSci.2022.194-200

学科分类：农业科学（综合）

来源: Journal of Livestock Science

【 摘 要 】

Dog erythrocytic membrane antigen plays a major role for determining blood group. Structural andmolecular characterization of erythrocytic membrane antigen improves the production of blood typing antisera, tostudy the auto antibody production in canine autoimmune haemolytic anemia. The proteins in the lipid domainarranged from the inside of the erythrocyte to the outside. The integral membrane proteins include membraneprotein 3 visible in Coomassie Brilliant Blue-stained polyacrylamide gels. The erythrocyte cytoskeleton consists ofspectrin, ankyrin, actin and protein 4.1 form a filamentous network under the lipid bilayer of erythroctic membrane.Most characteristic bands are associated with the CO =NH group referred to as amide A have NH stretching mostlyfound at 3500 cm-1 wave number. The amide B had NH stretching found at the region of 3100 cm -1 and amide I &III were used to estimate the secondary structure of proteins. Amide I mode which ranges from 1580 cm-1to 1700cm-1and very sensitive to the backbone conformation and not affected by the side chains. Amide I band can be deconvoluted with various sub-bands which directly correlate with various secondary structures The good intensitysharp peak at the level of 1468 cm-1and 2034.694 cm-1 were taken for 2D analysis the CCD cts scale bar showsdifferences between DEA1.1 positive and negative dog erythrocytic membrane antigen.

【 授权许可】

Unknown