Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay

The Journal of Veterinary Medical Science

article

Illia AOTA¹  Chisato YATSUDA¹  Ei-ichi IZAWA¹ 

[1] Department of Psychology, Keio University;Japan Society for Promotion of Science

关键词: bird;    corticosterone;    enzyme-linked immunoassay;    glucocorticoid;    stress;   

DOI  :  10.1292/jvms.22-0383

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal’s stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported. We aimed to measure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10–60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.

【 授权许可】

Unknown