Rapid determination of pathogens in mastitic milk of dairy cows using Gram staining

The Journal of Veterinary Medical Science

article

Naoki SUZUKI¹  Sohei KANEKO²  Naoki ISOBE¹ 

[1] Graduate School of Integrated Sciences for Life, Hiroshima University;Higashi-Hiroshima Veterinary Clinical Center

关键词: dairy cow;    diagnosis;    Gram staining;    mastitis;   

DOI  :  10.1292/jvms.21-0631

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

This study aimed to determine whether causative pathogens in mastitic milk can be determined by Gram staining after the centrifugation of milk. Gram staining was performed using unconcentrated and concentrated milk cells. Using this method, we found that the background of microscopic image of unconcentrated milk cells was complex and bacteria were difficult to detect. In contrast, the background of the smears in the concentrated milk cells was translucent, and bacterial and somatic cells were clearly visible. The sensitivity and specificity of the Gram staining of concentrated milk cells were 84.4% and 86.0% and 50.0% and 94.5% for the detection of gram-positive and gram-negative bacteria, respectively. The presented method provides a simple and inexpensive means of determining mastitis-causing pathogens.

【 授权许可】

Unknown