Jordan Journal of Biological Sciences | |
Transcriptional Impact of E-cadherin Loss on Embryonic Stem Cells | |
article | |
Hani Alotaibi1  | |
[1] Izmir Biomedicine and Genome Center;Izmir International Biomedicine and Genome Institute, Dokuz Eylül University | |
关键词: E-cadherin; Epithelial to Mesenchymal Transition; EMT; Embryonic Stem Cells; Gene replacement.; | |
DOI : 10.54319/jjbs/140524 | |
学科分类:生物科学(综合) | |
来源: Hashemite University, Deanship of Research and Higher Studies | |
【 摘 要 】
In embryonic stem cells, E-cadherin plays a crucial role in sustaining the pluripotent state; this is achieved by the simultaneous presence of active core pluripotency transcriptional network, proper cell-cell adhesion, and an undifferentiatedstate chromatin signature. In contrast, N-cadherin is linked to a more differentiated cell state but can support pluripotency if expressed as a knock-in allele from the E-cadherin locus. This study describes the N-cadherin ki/ki embryonic stem cells, which lack E-cadherin expression, to identify transcriptional changes distinct from the known changes observed in Ecadherin knockout embryonic stem cells. As a result, a remarkable similarity in the expression profiles of N-cadherin ki/ki and wild-type embryonic stem cells was observed. Further analysis of the slight differences revealed significant alterations in several biological processes such as chromatin organization and epithelial-mesenchymal transition. The findings presented here shed light on a new aspect of E-cadherin biology in embryonic stem cells and lay the foundations for comprehensive understanding of E-cadherin's functional relevance beyond the prominent role in maintaining pluripotency.
【 授权许可】
CC BY
【 预 览 】
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RO202306300002013ZK.pdf | 1125KB | download |