Morphological Identification of Mycorrhizal Fungi Isolated from Native Orchid in Indonesia

Jordan Journal of Biological Sciences

article

Mahfut¹ 

[1] Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Lampung

关键词: Ceratobasidium;    mycorrhiza;    morphological analysis;    Phalaenopsis;    Indonesia;   

DOI  :  10.54319/jjbs/140523

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Moth orchid [Phalaenopsis amabilis (L.) Blume] is a species of native orchid from Indonesia. The association of this orchid with mycorrhizal fungi in nature is as a biocontrol agent. In a previous study, one Ceratobasidium isolate from Yogyakarta, Indonesia was successfully identified based on rDNA-ITS molecular analysis. This study aimed to identify these isolates based on morphological analysis to complement other identification data, namely anatomy and molecular. Verification morphological analysis is carried out by observing macroscopic and microscopic characteristics, as well as observing peloton. The results of showed that the characterization of Yogyakarta isolates had similarities with Ceratobasidium. These character equations include colony color, cell length, core number, and cell width. The examination of orchid roots also showed the presence of pelotons in the cortical cells. This study confirms that the fungal isolates of orchid mycorrhiza from Yogyakarta, Indonesia were Ceratobasidium based on morphological and molecular analysis. This research is one of the efforts to preserve native orchids in Indonesia using myccorhiza fungi as a biocontrol agent. This study is the first to report regarding Ceratobasidium isolated from native orchids in Indonesia based on morphological analysis.

【 授权许可】

CC BY