Genetic improvement of Pseudomonas aeruginosa and Bacillus cereus for controlling root knot nematode and two weeds under laboratory conditions

Jordan Journal of Biological Sciences

article

Shereen AH. Mohamed¹  Hoda H. Ameen²  Usama S. Elkelany²  Mona A. ElWakeel³  Mostafa MA. Hammam²  Gaziea M. Soliman² 

[1] Microbial Genetics Department, National Research Centre;Plant Pathology Department National Research Centre;Botany Department, National Research Centre

关键词: Protoplast fusion;    SDS-PAGE;    Pseudomonas aeruginosa;    Bacillus cereus;    Biocontrol;    Meloidogyne incognita;    Echinochloa;   

DOI  :  10.54319/jjbs/140428

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Plant parasitic nematodes and weeds are among the many biotic stresses that crops production suffers from during their growing season. Their management relies mainly on chemical pesticides. To decrease the extent of environment degradation and hazards to human health and livestock due to the prolonged use of these chemicals, biological control using soil microorganisms is considered as a new ecofriendly and efficient control method. The main objective of this work was to improve the inhibition of two local bacterial strains, Pseudomonas aeruginosa and Bacillus cereus, against root knot nematode and weed seeds germination. To achieve this goal, protoplast fusion experiments were performed to gather all their properties in bacterial fusants and increase production of such toxic compounds. The results showed that P. aeruginosa was Rifampicin (Rif) resistance, but B. cereus was sensitive. In contrast to this B. cereus was Neomycin (Nm) resistance but P. aeruginosa was sensitive. A total of 40 fusants derived from the protoplast fusion experiments were selected by antibiotic resistance markers. SDS-PAGE analysis of the proteins confirmed that six recombinants acquired and expressed many specific protein bands from their parental strains. Three fusants, No. F7, F20 and F35, were selected and evaluated for their nematicidal potential in comparison with their parent against root knot nematode Meloidogyne incognita J and Echinochloa 2crus-galli and Portulaca oleracea seeds germination during in vitro experiments. Data showed that the fusants exhibited more antagonistic effects than their parents. After 72hrs of exposure, the three fusants caused 80.6, 96.5 and 97.7% mortality as compared to control, while the % mortality after the same duration by P. aeruginosa, B. cereus singly and combined resulted in 52.2, 65.9 and 48.8%, respectively as compared to control. Furthermore, the three fusants completely inhibited the germination of P. oleracea seeds and resulted in small radicals in E. crus-galli seeds as compared to control. These fusants show great potential to be selected as possible potential biopesticide.

【 授权许可】

CC BY