Employment of Somatic Embryogenesis as a tool for Rescuing Imperiled Narcissus tazetta L. Growing wild in Jordanian Environment.

Jordan Journal of Biological Sciences

article

Tamara M. Al-Zghoul¹  Rida A. Shibli¹  Tamara S. Qudah²  Reham W. Tahtamouni³  Nasab Rawshdeh⁴ 

[1] Department of Horticulture and Agronomy, Faculty of Agriculture, University of Jordan;Hamdi Mango Center for Scientific Research ,(HMCSR), University of Jordan;Department of Biotechnology, Faculty of Agricultural technology, Al Balqa- Applied University;National Center for Agricultural Research and Extension

关键词: Callus;    Embryogenesis;    Narcissus tazetta;    Somatic embryo.;   

DOI  :  10.54319/jjbs/140323

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Somatic embryogenesis was used as a tool for micropropagation of wild Narcissus tazetta plants exposing to overcollection and rapidly changed environmental conditions. In the growth regulators experiment, somatic embryogenesis was successfully induced in all treatments except for explants grown in the control treatment (Murashig and Skoog (MS) hormone-free medium). Meanwhile, the highest value for the number of somatic embryos/ callus segment (441) was -1obtained into MS media supplemented with 0.2 mg l 6-(gamma, gamma- Dimethylallylamino) purine (2iP) under dark -1 conditions. Moreover, sucrose at 30g L was the best sugar source resulting in higher number of somatic embryos compared to the other sugar treatments. The highest shoot development rate from somatic embryos was (191.99/ non-embryonic calli -1segment) recorded in cultures grown on MS media plus 0.5 mg l 2iP. Maximum bulbet size (1.6 cm diameter) was recorded in plantlets kept onto hormone-free MS hormone free media for 6 weeks before acclimatization, while less durations resulted in smaller bulbet size. Well developed in vitro plantlets were acclimatized successfully with high survival percentage of (95%). The acclimatized plants were normal and did not show any morphological abnormalities.

【 授权许可】

CC BY