Histopathological Alterations in the Gills and Liver of Clarias Gariepinus Juveniles Exposed to Acute Concentrations of Anogeissus Leiocarpus

Jordan Journal of Biological Sciences

article

Bala Sambo Audu¹  Idris Audu Wakawa²  Omirinde Jamiu Oyewole³  Ponwa Zingfa Changdaya¹ 

[1] Hydrobiology and Fisheries Unit, Department of Zoology, University of Jos;Department of Biology, Umar Suleiman College of Education;Department of Anatomy, Faculty of Veterinary Medicine, University of Jos. Nigeria.

关键词: Acute toxicity;    Histopathology;    Anogeissus leiocarpus;    Clarias gariepinus;   

DOI  :  10.54319/jjbs/140320

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Over the years, people have used the stem bark extract of Anogeissus leiocarpus in traditional tanneries as a native agent in tendering hide and skin. The wastes from the processing plant are washed into aquatic environment and cause pollution. The acute toxicity (96hr-LC50) of the aqueous crude stem extract of A. leiocarpus on behaviour and histopathology of gills and liver of Clarias gariepinus juveniles (average weight 42.00±0.05 g and average total length 27.83±0.71 cm) were investigated in a static non-renewable bioassay to ascertain its toxicity. A total of five concentrations of aqueous crude stem extract of A. leiocarpus (450.00, 400.00, 350.00, 300.00, and 250.00 mg/L) and a control (0.00 g/L) were used. Ten (10) juveniles were stocked in each tank with dimension of 50x30x25 cm. Of the one hundred and twenty (120) mixed sex C. gariepinus juveniles (in duplicate replication) used, mortalities of 100, 70, 50, 20, and 10% were recorded in concentrations 450.00, 400.00, 350.00, 300.00, and 250.00 mg/L respectively while control recorded 0% after 96 hr. The 96 hr. LC50 of A. leiocarpus on C. gariepinus resulted in 353.77 mg/L characterized by upper and lower confidence limits of 390.27 and 320.69 mg/L respectively. There was marked variation in the water quality parameters (total alkalinity and free carbon dioxide) in all the test tanks compared with the control. The behavioural signs exhibited by C. gariepinus exposed to concentrated grades of the plant material were erratic swimming, loss of stability, spiral movement, air gulping, restlessness and settling on tank bottom. Histopathological alterations such as lamellar vascular congestion, lamellar clubbing and partial to complete inter-lamellar space occlusion were recorded in the gills of Clarias gariepinus exposed to the graded concentrations of the extract. The liver of the catfish showed dose-related hepatic lesions such as portal congestion, periportal cellular degeneration and cellular infiltration. This study shows that 300.00 to 450.00 mg/L of A. leiocarpus is toxic to fish's health. Hence its indiscriminate disposal into aquatic environment should be discouraged or totally avoided to avert death of aquatic animals.

【 授权许可】

CC BY