Long-term modification of gut microbiota by broad-spectrum antibiotics improves stroke outcome in rats

Stroke and Vascular Neurology

article

Chang Liu¹  Xi Cheng¹  Shanshan Zhong¹  Zhouyang Liu¹  Fangxi Liu¹  Xinyu Lin¹  Yinan Zhao¹  Meiting Guan¹  Ting Xiao³  Jukka Jolkkonen⁵  Ying Wang⁶  Chuansheng Zhao¹ 

[1] Department of Neurology , The First Hospital of China Medical University;The Stroke Center , The First Hospital of China Medical University;Department of Dermatology , The First Hospital of China Medical University;Key Laboratory of Immunodermatology , Ministry of Health, Ministry of Education;A.I. Virtanen Institute for Molecular Sciences , University of Eastern Finland;Department of Neurology , First Affiliated Hospital of Dalian Medical University

关键词: Stroke;    Stroke Rehabilitation;    Cerebral Infarction;   

DOI  :  10.1136/svn-2021-001231

学科分类：社会科学、人文和艺术（综合）

来源: BMJ Publishing Group

【 摘 要 】

Background The brain-gut axis is a major regulator of the central nervous system. We investigated the effects of treatment with broad-spectrum antibiotics on gut and brain inflammation, infarct size and long-term behavioral outcome after cerebral ischemia in rats.Methods Rats were treated with broad-spectrum antibiotics (ampicillin, vancomycin, ciprofloxacin, meropenem and metronidazole) for 4 weeks before the endothelin-1 induced ischemia. Treatment continued for 2 weeks until the end of behavioral testing, which included tapered ledged beam-walking, adhesive label test and cylinder test. Gut microbiome, short-chain fatty acids and cytokine levels were measured together with an assessment of infarct size, neuroinflammation and neurogenesis.Results The results revealed that the antibiotics exerted a clear impact on the gut microbiota. This was associated with a decrease in systemic and brain cytokine levels, infarct size and apoptosis in the perilesional cortex and improved behavioral outcome.Conclusion Our results highlighted the significant relationship between intestinal microbiota and beneficial neuro-recovery after ischemic stroke.

【 授权许可】

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