Lysophosphatidylcholine acyltransferase 1 is involved in the regulation of exosome secretion and uptake in colorectal cancer cells

Biocell

article

HAIZHENG LIU¹  SHAOFEI CHANG² 

[1]Anorectal Surgery, Yuncheng City Central Hospital

[2]Department of Gastrointestinal and Pancreatic Surgery, Shanxi Provincial People’s Hospital

关键词: LPCAT1;    Exosomes;    Colorectal cancer;   

DOI  :  10.32604/biocell.2021.015340

学科分类：仪器

来源: Biocell

【 摘 要 】

Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is a phospholipid acyltransferase that promotesphospholipid synthesis and plasma membrane reconstruction. Exosomes play an important role in tumor metastasis.The release and uptake of exosomes are key steps of their functions and depend on plasma membrane fusion andplasma membrane receptors, respectively. The purpose of this study was to explore whether LPCAT1-induced plasmamembrane remodeling would change the secretion and uptake behavior of exosomes in tumor cells. We firstconfirmed the abnormally high expression of LPCAT1 in colorectal cancer cells by quantitative real-time PCR (qPCR)and Western blot analysis. Then, SW620 cells were used as exosome source cells, and SW480 cells were used asexosome receiver cells. Exosomes from SW620 cells could effectively promote the migration of SW480 cells. WhenLPCAT1 expression was reduced via siRNA knockdown in the source cells, the secretion of exosomes wasdownregulated, thus weakening the pro-migratory effects of exosomes on target cells. Conversely, when LPCAT1 wasknocked down in target cells, the uptake of exosomes in target cells also decreased sharply. These results undoubtedlyrevealed that LPCAT1 is functionally associated with the release and internalization of exosomes in colorectal cancercells and could affect the paracrine effects of exosomes, preliminarily extending the classical metabolic function ofLPCAT1 to exosome-related pathways.

【 授权许可】

CC BY