Serotonin increases interleukin-6 synthesis in human vascular smooth muscle cells

Article

关键词: PROTEIN-KINASE-C;    HUMAN CORONARY-ARTERIES;    NF-KAPPA-B;    ATHEROSCLEROTIC LESIONS;    CARDIOVASCULAR-DISEASE;    MYOCARDIAL-INFARCTION;    TRANSCRIPTION FACTOR;    GENE TRANSCRIPTS;    GROWTH-FACTOR;    RAT AORTA;   

DOI  :  10.1161/01.CIR.102.20.2522

来源: SCIE

【 摘 要 】

Background-Interleukin-6 (IL-6) is a key molecule in chronic inflammation and has been implicated in the progression of atherosclerosis. Serotonin (5-hydroxytryptamine; 5-HT) causes vascular contraction and proliferation, but its role in atherogenesis has not been clarified. We investigated the effects of 5-HT on IL-6 synthesis in human vascular smooth muscle cells (VSMCs). Methods and Results-IL-6 levels in the culture medium of VSMCs were determined by ELISA. IL-6 mRNA accumulation was determined by use of a Quantikine mRNA colorimetric quantification kit. NF-kappaB activation was tested by gel retardation assay. 5-HT induced IL-6 production by VSMCs in a time- and dose-dependent manner, with increased IL-6 mRNA accumulation and nuclear factor-kappaB activation. The effect of 5-HT on IL-6 production was significantly inhibited by the 5-HT2 receptor antagonist ketanserin and the selective 5-HT2A receptor antagonist sarpogrelate. Conversely, the 5-HT2 receptor agonist alpha -methyl-5-HT increased IL-6 production. The protein kinase C (PKC) inhibitor calphostin C, but not the protein kinase A inhibitor KT5720, suppressed 5-HT-induced IL-6 production. The effect of 5-HT was also abolished in PKC-depleted VSMCs after pretreatment with phorbol 13-myristate 13-acetate for 24 hours. Conclusions-5-HT acts on 5-HT2A receptors and increases IL-6 synthesis in human VSMCs at least partially through a PKC-dependent pathway. These results suggested that 5-HT may contribute to inflammatory activation of the vessels during atherogenesis.

【 授权许可】

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