Serum from patients with acute coronary syndromes displays a proapoptotic effect on human endothelial cells - A possible link to pan-coronary syndromes

Article

关键词: ACUTE MYOCARDIAL-INFARCTION;    ANGIOGRAPHIC MORPHOLOGY;    ATHEROSCLEROTIC PLAQUES;    UNSTABLE ANGINA;    HEART-FAILURE;    APOPTOSIS;    THROMBOSIS;    PATHOGENESIS;    PROCOAGULANT;    RUPTURE;   

DOI  :  10.1161/01.CIR.0000045665.57256.86

来源: SCIE

【 摘 要 】

Background-Endothelial apoptosis of atherosclerotic lesions is a possible determinant for the stable-to-vulnerable plaque transition. Recent data support the notion that plaque activation may be a pan-coronary process, advocating the existence of circulating triggers. Methods and Results-Serum from 40 healthy subjects (group 1) and 73 patients with stable angina (n=32; group 2) or acute coronary syndromes (n=41; group 3) was incubated with human umbilical vein endothelial cells. The percentage of apoptosis by flow cytometry and Fas, Bax, and Bcl-2 protein expression by immunoblotting were evaluated at entry in patients and control subjects and repeated after 12 months in group 3. At baseline, apoptotic nuclei were higher in group 3 (14 +/- 6%) than in group 2 (3.3 +/- 1.8%) and group 1 (1.35 +/- 0.8%) (P<0.001). Fas and Bcl-2 were increased in group 3 with respect to groups 1 and 2 (P<0.01). Coincubation of group 3 serum with anti-tumor necrosis factor-a and anti-interleukin-6 monoclonal antibodies did not affect the human umbilical vein endothelial cell apoptotic process, whereas addition of Trolox decreased apoptosis to <50%. The percentage of apoptosis in group 3 significantly correlated to the numbers of coronary complex lesions at angiography (r=0.58, P<0.0005). In group 3, apoptosis and the Bax/Bcl-2 ratio decreased at 1 year (P<0.0001, P<0.05 respectively). Conclusions-Serum from patients with acute coronary syndromes displays a proapoptotic effect on human endothelial cells, supporting the theory of the existence of circulating triggers potentially able to activate atherosclerotic lesions.

【 授权许可】

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