C-reactive protein increases plasminogen activator inhibitor-1 expression and activity in human aortic endothelial cells

Article

关键词: GENE-EXPRESSION;    RISK-FACTOR;    ARTERIAL;    TYPE-1;    PAI-1;    INTERLEUKIN-6;    INFLAMMATION;    RECEPTOR;    DISEASE;    PLASMA;   

DOI  :  10.1161/01.CIR.0000052617.91920.FD

来源: SCIE

【 摘 要 】

Background-Inflammation plays a pivotal role in atherosclerosis. In addition to being a risk marker for cardiovascular disease, much recent data suggest that C-reactive protein (CRP) promotes atherogenesis via effects on monocytes and endothelial cells. The metabolic syndrome is associated with significantly elevated levels of CRP. Plasminogen activator inhibitor-1 (PAI-1), a marker of atherothrombosis, is also elevated in the metabolic syndrome and in diabetes, and endothelial cells are the major source of PAI-1. However, there are no studies examining the effect of CRP on PAI-1 in human aortic endothelial cells (HAECs). Methods and Results-Incubation of HAECs with CRP results in a time- and dose-dependent increase in secreted PAI-1 antigen, PAI-1 activity, intracellular PAI-1 protein, and PAI-1 mRNA. CRP stabilizes PAI-1 mRNA. Inhibitors of endothelial NO synthase, blocking antibodies to interleukin-6 and an endothelin-1 receptor blocker, fail to attenuate the effect of CRP on PAI-1. CRP additionally increased PAI-1 under hyperglycemic conditions. Conclusions-This study makes the novel observation that CRP induces PAI-1 expression and activity in HAECs and thus has implications for both the metabolic syndrome and atherothrombosis.

【 授权许可】

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