c-Jun N-Terminal Kinase 2 Deficiency Protects Against Hypercholesterolemia-Induced Endothelial Dysfunction and Oxidative Stress

Article

关键词: NITRIC-OXIDE;    XANTHINE-OXIDASE;    SIGNAL-TRANSDUCTION;    PEPTIDE INHIBITOR;    UP-REGULATION;    JNK2;    ATHEROSCLEROSIS;    PEROXYNITRITE;    INFLAMMATION;    ACTIVATION;   

DOI  :  10.1161/CIRCULATIONAHA.108.765032

来源: SCIE

【 摘 要 】

Background - Hypercholesterolemia-induced endothelial dysfunction due to excessive production of reactive oxygen species is a major trigger of atherogenesis. The c-Jun-N-terminal kinases (JNKs) are activated by oxidative stress and play a key role in atherogenesis and inflammation. We investigated whether JNK2 deletion protects from hypercholesterolemia-induced endothelial dysfunction and oxidative stress. Methods and Results - Male JNK2 knockout (JNK2(-/-)) and wild-type (WT) mice (8 weeks old) were fed either a high-cholesterol diet (HCD; 1.25% total cholesterol) or a normal diet for 14 weeks. Aortic lysates of WT mice fed a HCD showed an increase in JNK phosphorylation compared with WT mice fed a normal diet (P < 0.05). Endothelium-dependent relaxations to acetylcholine were impaired in WT HCD mice (P < 0.05 versus WT normal diet). In contrast, JNK2(-/-) HCD mice did not exhibit endothelial dysfunction (96 +/- 5% maximal relaxation in response to acetylcholine; P < 0.05 versus WT HCD). Endothelium-independent relaxations were identical in all groups. A hypercholesterolemia-induced decrease in nitric oxide (NO) release of endothelial cells was found in WT but not in JNK2(-/-) mice. In parallel, endothelial NO synthase expression was upregulated only in JNK2(-/-) HCD animals, whereas the expression of antioxidant defense systems such as extracellular superoxide dismutase and manganese superoxide dismutase was decreased in WT but not in JNK2(-/-) HCD mice. In contrast to JNK2(-/-) mice, WT HCD displayed an increase in O-2(-) and ONOO- concentrations as well as nitrotyrosine staining and peroxidation. Conclusions - JNK2 plays a critical role as a mediator of hypercholesterolemia-induced endothelial dysfunction and oxidative stress. Thus, JNK2 may provide a novel target for prevention of vascular disease and atherosclerosis. (Circulation. 2008; 118: 2073-2080.)

【 授权许可】

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