Impaired VE-cadherin/beta-catenin expression mediates endothelial cell degeneration in dilated cardiomyopathy

Article

关键词: SIGNAL-TRANSDUCTION PATHWAY;    ISCHEMIC CARDIOMYOPATHY;    IN-VIVO;    ANGIOGENESIS;    SURVIVAL;    MICE;    ANGIOPOIETIN-1;    MECHANISMS;    APOPTOSIS;    TIE2;   

DOI  :  10.1161/01.CIR.0000091085.12422.19

来源: SCIE

【 摘 要 】

Background - The cross-talk between vascular endothelial growth factor ( VEGF)- A, angiopoietin (Ang), and VE-cadherin coregulates endothelial cell (EC) survival. Cardiac expression of VEGF-A but not its receptor KDR is blunted in dilated cardiomyopathy (DCM). Whether VE-cadherin/Ang function is affected in DCM is unknown. Methods and Results - The myocardial expression of VE-cadherin/beta-catenin, Ang-1, Ang-2, and their receptor Tie-2 was examined in DCM, ischemic cardiomyopathy (ICM), and in control subjects through the use of real-time RT-PCR, Western blotting, and immunocytochemistry. EC degeneration was quantified by TEM. RNA interference against VE-cadherin and VEGF deprivation and stimulation were applied to cultured DCM myocardium and human microvascular ECs to examine the interplay between VEGF, VE-cadherin/beta-catenin, and Ang-2. Analysis of tissue sections with similar rates of EC degeneration in both patient groups showed that VE-cadherin/beta-catenin expression was downregulated in DCM only (P < 0.05). Although Ang-1 was not changed, Ang-2 expression was downregulated and Tie-2 protein expression was upregulated both in DCM and ICM ( P < 0.05). The ratio of degenerated to normal ECs was significantly higher in DCM versus ICM (P < 0.05). Targeted VE-cadherin gene silencing in cultured human ECs resulted in similar degenerative effects observed in myocardial ECs of DCM patients. In vitro experiments indicated that VE-cadherin/β-catenin expression is independent of VEGF. Conclusions - These results indicate for the first time that the EC survival is impaired in myocardium of patients with DCM involving VE-cadherin/β-catenin, probably independent of VEGF. Targeting VE-cadherin might be of benefit to counteract the selective EC pathology in DCM.

【 授权许可】

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