Rate dependence and regulation of action potential and calcium transient in a canine cardiac ventricular cell model | |
Article | |
关键词: EPICARDIAL BORDER ZONE; SARCOPLASMIC-RETICULUM; PROTEIN-KINASE; CA2+ RELEASE; PHOSPHOLAMBAN PHOSPHORYLATION; ENDOCARDIAL MYOCYTES; RYANODINE RECEPTORS; OUTWARD CURRENT; LOCAL-CONTROL; HEART-CELLS; | |
DOI : 10.1161/01.CIR.0000147231.69595.D3 | |
来源: SCIE |
【 摘 要 】
Background - Computational biology is a powerful tool for elucidating arrhythmogenic mechanisms at the cellular level, where complex interactions between ionic processes determine behavior. A novel theoretical model of the canine ventricular epicardial action potential and calcium cycling was developed and used to investigate ionic mechanisms underlying Ca2+ transient (CaT) and action potential duration (APD) rate dependence. Methods and Results - The Ca2+/calmodulin-dependent protein kinase (CaMKII) regulatory pathway was integrated into the model, which included a novel Ca2+-release formulation, Ca2+ subspace, dynamic chloride handling, and formulations for major ion currents based on canine ventricular data. Decreasing pacing cycle length from 8000 to 300 ms shortened APD primarily because of I-Ca(L) reduction, with additional contributions from I-tol, I-NaK, and late I-Na. CaT amplitude increased as cycle length decreased from 8000 to 500 ms. This positive rate - dependent property depended on CaMKII activity. Conclusions - CaMKII is an important determinant of the rate dependence of CaT but not of APD, which depends on ion-channel kinetics. The model of CaMKII regulation may serve as a paradigm for modeling effects of other regulatory pathways on cell function.
【 授权许可】
Free