Gene therapy of endothelial nitric oxide synthase and manganese superoxide dismutase restores delayed wound healing in type 1 diabetic mice

Article

关键词: GROWTH-FACTOR EXPRESSION;    NECROSIS-FACTOR-ALPHA;    NAD(P)H OXIDASE;    VASCULAR SUPEROXIDE;    CORONARY-ARTERIES;    HYDROGEN-PEROXIDE;    DEFICIENT MICE;    NADPH OXIDASE;    SKIN REPAIR;    NO SYNTHASE;   

DOI  :  10.1161/01.CIR.0000137969.87365.05

来源: SCIE

【 摘 要 】

Background-Nitric oxide (NO) deficiency contributes to diabetic wound healing impairment. The present study tested the hypothesis that increased cutaneous superoxide (O-2(-)) levels in type 1 diabetic mice cause NO deficiency and delayed wound healing. Methods and Results-Wound healing was markedly delayed in streptozotocin-induced type 1 diabetic mice compared with the normal controls. There were significantly reduced levels of endothelial NO synthase (eNOS) protein and constitutive NOS activity in diabetic wounds, whereas O-2(-) levels were markedly increased. A single regimen of cutaneous gene therapy of eNOS or manganese superoxide dismutase (MnSOD) restored such healing delay, with a concomitant suppression of wound O-2(-) levels and augmentation of both eNOS protein and constitutive NOS activity. Gene therapy of MnSOD also increased cutaneous MnSOD activity. Cutaneous O-2(-) levels were also increased in Ins2(Akita) diabetic mice. In vitro glucose treatment of cutaneous tissues from normal mice for 24 hours increased O-2(-) levels in a concentration-dependent manner. The enhanced cutaneous O-2(-) levels induced by high glucose in both normal and diabetic mice were abolished by the NADPH oxidase inhibitor apocynin and the protein kinase C inhibitor chelerythrine. Furthermore, ex vivo gene transfer of dominant-negative HA-tagged N17Rac1, which inhibits NADPH oxidase subunit Rac1, significantly inhibited cutaneous O-2(-) formation induced by high glucose in both normal and Ins2Akita diabetic mice. Conclusions-These results indicate that hyperglycemia augments cutaneous O-2(-) levels, at least in part, via NADPH oxidase and protein kinase C pathways, resulting in impaired wound healing in type 1 diabetic mice. Gene therapy strategies aimed at restoring cutaneous NO bioavailability may provide an effective means to ameliorate delayed diabetic wound healing.

【 授权许可】

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