G(13)-Mediated Signaling Pathway Is Required for Pressure Overload-Induced Cardiac Remodeling and Heart Failure

Article

关键词: BACTERIAL ARTIFICIAL CHROMOSOMES;    JUN NH2-TERMINAL KINASE;    ANGIOTENSIN-II;    MYOCARDIAL HYPERTROPHY;    MECHANICAL-STRESS;    GENE-EXPRESSION;    TRANSCRIPTION FACTOR;    CONTRACTILE FAILURE;    G-PROTEINS;    ACTIVATION;   

DOI  :  10.1161/CIRCULATIONAHA.112.109256

来源: SCIE

【 摘 要 】

Background-Cardiac remodeling in response to pressure or volume overload plays an important role in the pathogenesis of heart failure. Various mechanisms have been suggested to translate mechanical stress into structural changes, one of them being the release of humoral factors such as angiotensin II and endothelin-1, which in turn promote cardiac hypertrophy and fibrosis. A large body of evidence suggests that the prohypertrophic effects of these factors are mediated by receptors coupled to the G(q/11) family of heterotrimeric G proteins. Most G(q/11)-coupled receptors, however, can also activate G proteins of the G(12/13) family, but the role of G(12/13) in cardiac remodeling is not understood. Methods and Results-We use siRNA-mediated knockdown in vitro and conditional gene inactivation in vivo to study the role of the G(12/13) family in pressure overload-induced cardiac remodeling. We show in detail that inducible cardiomyocyte-specific inactivation of the alpha subunit of G(13), G alpha(13), does not affect basal heart function but protects mice from pressure overload-induced hypertrophy and fibrosis as efficiently as inactivation of G alpha(q/11). Furthermore, inactivation of G alpha(13) prevents the development of heart failure up to 1 year after overloading. On the molecular level, we show that G alpha(13), but not G alpha(q/11), controls agonist-induced expression of hypertrophy-specific genes through activation of the small GTPase RhoA and consecutive activation of myocardin-related transcription factors. Conclusion-Our data show that the G(12/13) family of heterotrimeric G proteins is centrally involved in pressure overload-induced cardiac remodeling and plays a central role in the transition to heart failure. (Circulation. 2012;126:1972-1982.)

【 授权许可】

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