Smooth muscle cells in human atherosclerotic plaques express the fractalkine receptor CX(3)CR1 and undergo chemotaxis to the CX3C chemokine fractalkine (CX3CL1)

Article

关键词: MONOCYTE CHEMOATTRACTANT PROTEIN-1;    CORONARY-ARTERY DISEASE;    LESION FORMATION;    DEFICIENT MICE;    IN-VIVO;    INFLAMMATION;    ACTIVATION;    ADHESION;    PATHWAY;    INJURY;   

DOI  :  10.1161/01.CIR.0000097119.57756.EF

来源: SCIE

【 摘 要 】

Background-Chemokines are important mediators of inflammatory cell recruitment that play a significant role in atherosclerosis. Fractalkine (CX(3)CL1) is an unusual membrane-bound chemokine that mediates chemotaxis through the CX(3)CR1 receptor. Recently, functional polymorphisms in the human CX3CR1 gene have been described that are associated with coronary artery disease. Methods and Results-We investigated the expression of the CX3C chemokine fractalkine and its receptor CX(3)CR1 in human coronary artery plaques by immunocytometry. We show that a subset of mononuclear cells expresses high levels of fractalkine in human coronary atherosclerotic plaques and that smooth muscle cells within the neointima express the fractalkine receptor CX(3)CR1. There is a positive correlation between the number of fractalkine-expressing cells and the number of CX(3)CR1-positive cells in human atherosclerotic plaques (r=0.70, n=15 plaques). Furthermore, we demonstrate that cultured vascular smooth muscle cells express the CX(3)CR1 receptor and undergo chemotaxis to fractalkine that can be inhibited by G protein inactivation by pertussis toxin. Conclusions-These results suggest that in human atherosclerosis, fractalkine, rather than mediating inflammatory cell recruitment, can act as a mediator of smooth muscle cell migration.

【 授权许可】

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