Pitavastatin downregulates expression of the macrophage type B scavenger receptor, CD36

Article

关键词: ACTIVATED PROTEIN-KINASE;    COA REDUCTASE INHIBITORS;    COENZYME-A REDUCTASE;    LOW-DENSITY-LIPOPROTEIN;    NITRIC-OXIDE SYNTHASE;    GENE-EXPRESSION;    PPAR-GAMMA;    OXIDIZED-LDL;    DIFFERENTIATION;    CELLS;   

DOI  :  10.1161/01.CIR.0000112576.40815.13

来源: SCIE

【 摘 要 】

Background - Pitavastatin (NK-104) is a novel inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme for cholesterol biosynthesis. In clinical trials, pitavastatin has been shown to significantly decrease serum LDL cholesterol and triglyceride levels and increase HDL cholesterol. Scavenger receptor - mediated accumulation of oxidized LDL ( OxLDL) - derived cholesteryl ester is considered to be a critical step in the development of atherosclerotic foam cell formation. We studied the effect of pitavastatin on CD36 ( a class B scavenger receptor) expression by murine macrophages. Methods and Results - Treatment of J774 cells and murine peritoneal macrophages with pitavastatin decreased CD36 mRNA expression in a dose-dependent manner. Decreased CD36 mRNA was associated with decreased CD36 cell surface protein expression in human THP-1 cells and human monocyte-derived macrophages. Pitavastatin also reduced the increase in CD36 mRNA, cell surface protein, and binding/uptake of OxLDL induced by peroxisome proliferator activated receptor-gamma (PPARgamma) ligands and/or OxLDL. Pitavastatin did not alter the half-life of CD36 mRNA, which suggests pitavastatin downregulates CD36 expression by reducing CD36 transcription. In addition, pitavastatin significantly decreased PPARgamma mRNA and protein expression. Finally, pitavastatin increased p44/42 mitogen-activated protein kinase activity and PPARgamma phosphorylation and increased the ratio of phosphorylated PPARgamma to nonphosphorylated PPARgamma. Conclusions - The present data demonstrate that pitavastatin prevents OxLDL uptake by macrophages through PPARgamma-dependent inhibition of CD36 expression and suggest that pitavastatin could modulate CD36-mediated atherosclerotic foam cell formation.

【 授权许可】

Free