Method of cell transplantation of intraarterial promoting the organization thrombus

Article

关键词: STENT-GRAFT PLACEMENT;    AORTIC DISSECTION;    ANEURYSM REPAIR;    BLOOD-CELLS;    MYOFIBROBLAST;    DIFFERENTIATION;    MANAGEMENT;   

DOI  :  10.1161/01.CIRCULATIONAHA.104.525071

来源: SCIE

【 摘 要 】

Background-Endovascular aortic repairs have been developed as less invasive treatments for aortic aneurysms. Some aneurismal cavities, however, remain without organization, causing a re-expansion of the aneurysms. We studied cell transplantation into the aneurismal sac to promote the organization of thrombus for the complete healing of aneurysms. Methods and Results-Skin fibroblasts and skeletal myoblasts were isolated from rats for cell transplantation. An intraarterial thrombus model was made by ligation of the carotid artery. Culture medium (medium group, n=11), collagen gel (gel group, n =11), fibroblasts with collagen gel (F group, n= 15), myoblasts with collagen gel (M group, n = 12), or mixture of fibroblasts and myoblasts with collagen gel (F+ M group, n = 14) were injected into the thrombus. After 28 days, histologically, the arterial lumens of the F and M groups were partly filled with fibrous tissues, whereas in the F+M group organization was almost completed and luminal sizes diminished. Immunohistochemical staining demonstrated that a-smooth muscle actin-positive cells were more abundantly contained in the organized area of the F+M group than in the other groups. We also analyzed cellular function in vitro with immunofluorescence; coculture of fibroblasts and myoblasts showed that the fraction of a-smooth muscle actin-positive fibroblasts increased. This phenomenon accounts for the rapid organization of thrombus in the F+M group in vivo. Conclusions-Cell transplantation accelerated thrombus organization. Especially, myoblasts enhanced differentiation of fibroblasts into myofibroblasts, contributing to rapid thrombus organization. Cell transplantation into unorganized spaces seems applicable to endovascular treatment of aneurysms.

【 授权许可】

Free